Effects of high and low level 1265 nm laser irradiation on HCT116 cancer cells

Anna Khokhlova; Igor Zolotovskii; Evgenia Pogodina; Yury Saenko; Dmitrii Stoliarov; Svetlana Vorsina; Andrei Fotiadi; Daria Liamina; Sergei Sokolovski; Edik Rafailov

doi:10.1117/12.2509529

7 March 2019 Effects of high and low level 1265 nm laser irradiation on HCT116 cancer cells

Anna Khokhlova, Igor Zolotovskii , Evgenia Pogodina, Yury Saenko, Dmitrii Stoliarov, Svetlana Vorsina, Andrei Fotiadi, Daria Liamina, Sergei Sokolovski, Edik Rafailov

Author Affiliations +

Proceedings Volume 10861, Mechanisms of Photobiomodulation Therapy XIV; 108610L (2019) https://doi.org/10.1117/12.2509529
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

The mechanism responsible for the oxidative stress due to photobiomodulation induced by 1265 nm laser is still unclear. Mitochondria are assumed to be the most probable acceptors of the 1265 nm laser irradiation. We study oxidative stress, mitochondrial potential, GSH, cell viability, DNA damage. We demonstrated that narrowband (highcoherent) and wideband lasers employed at the doses of 9.45 and 66.6-400 J/cm², respectively, induce a dose-dependent cell death, increase ROS level, disturb mitochondrial functioning and can damage DNA. Thus, the 1265 nm lasers can affect the HCT116 cells through mitochondrial damage. Energy density increase contributes to cell damaging without heating effects.

Conference Presentation

Citation Download Citation

Anna Khokhlova, Igor Zolotovskii , Evgenia Pogodina, Yury Saenko, Dmitrii Stoliarov, Svetlana Vorsina, Andrei Fotiadi, Daria Liamina, Sergei Sokolovski, and Edik Rafailov "Effects of high and low level 1265 nm laser irradiation on HCT116 cancer cells", Proc. SPIE 10861, Mechanisms of Photobiomodulation Therapy XIV, 108610L (7 March 2019); https://doi.org/10.1117/12.2509529

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available