Quantitative oblique back-illumination microscopy (qOBM) for stain-free tissue imaging with cycleGAN mode conversion can emulate H&E histology

Tanishq Abraham; Paloma C. Costa; Caroline E. Filan; Francisco Robles; Richard M. Levenson

doi:10.1117/12.3008530

3 April 2024 Quantitative oblique back-illumination microscopy (qOBM) for stain-free tissue imaging with cycleGAN mode conversion can emulate H&E histology

Tanishq Abraham, Paloma C. Costa, Caroline E. Filan, Francisco Robles, Richard M. Levenson

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Proceedings Volume 12933, Medical Imaging 2024: Digital and Computational Pathology; 1293311 (2024) https://doi.org/10.1117/12.3008530
Event: SPIE Medical Imaging, 2024, San Diego, California, United States

Abstract

Quantitative oblique back-illumination microscopy (qOBM) is a novel technology for label-free imaging of thick (unsectioned) tissue specimens, demonstrating high spatial resolution and 3-D capabilities. The grayscale contrast however, is unfamiliar to pathologist and histotechnicians without familiarization, limiting its adoption. We used deep learning techniques to convert qOBM into virtual H&E, observing successful conversion of both healthy and tumor thick (unsectioned) specimens. Transfer learning was demonstrated on a second collection of qOBM and H&E images of human astrocytoma specimens. With some improvement in robustness and generalizability, we anticipate that this approach can find clinical application.

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Tanishq Abraham, Paloma C. Costa, Caroline E. Filan, Francisco Robles, and Richard M. Levenson "Quantitative oblique back-illumination microscopy (qOBM) for stain-free tissue imaging with cycleGAN mode conversion can emulate H&E histology", Proc. SPIE 12933, Medical Imaging 2024: Digital and Computational Pathology, 1293311 (3 April 2024); https://doi.org/10.1117/12.3008530

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KEYWORDS

Tissues

Microscopy

Tumors

3D imaging standards

Data modeling

Education and training

In vivo imaging

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