Simultaneous mapping of refractive index and longitudinal modulus via dual-geometry Brillouin microscopy

Antonio Fiore; Giuliano Scarcelli

doi:10.1117/12.2577716

5 March 2021 Simultaneous mapping of refractive index and longitudinal modulus via dual-geometry Brillouin microscopy

Antonio Fiore, Giuliano Scarcelli

Proceedings Volume 11645, Optical Elastography and Tissue Biomechanics VIII; 1164508 (2021) https://doi.org/10.1117/12.2577716
Event: SPIE BiOS, 2021, Online Only

Abstract

Brillouin is a light-matter interaction that depends on several material properties, including the refractive index and the longitudinal modulus of the sample. We developed a technique that probes two different Brillouin scattering geometries in the same volume, allowing the retrieval of the index of refraction and the speed of the acoustic phonon in a direct manner. In biological samples, these two quantities are correlated respectively to the dry mass density and the longitudinal modulus. Coupling such measurement with a confocal microscope allows mapping of these properties for samples and in modalities unavailable with previous approaches.

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Antonio Fiore and Giuliano Scarcelli "Simultaneous mapping of refractive index and longitudinal modulus via dual-geometry Brillouin microscopy", Proc. SPIE 11645, Optical Elastography and Tissue Biomechanics VIII, 1164508 (5 March 2021); https://doi.org/10.1117/12.2577716

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KEYWORDS

Refractive index

Microscopy

Refraction

In vivo imaging

Phonons

Scattering

Spatial resolution

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