The effect of low-level laser therapy on severe acute respiratory syndrome coronavirus-2 infected cells

L. Mngwengwe; M. Y. Lugongolo; S. Ombinda-Lemboumba; Y. Ismail; P. Mthunzi-Kufa

doi:10.1117/12.3002453

12 March 2024 The effect of low-level laser therapy on severe acute respiratory syndrome coronavirus-2 infected cells

L. Mngwengwe, M. Y. Lugongolo, S. Ombinda-Lemboumba, Y. Ismail, P. Mthunzi-Kufa

Proceedings Volume 12826, Mechanisms of Photobiomodulation Therapy XVIII; 1282606 (2024) https://doi.org/10.1117/12.3002453
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

SARS-CoV-2 is a pandemic that claimed millions of lives, and to date, there has been no identifiable cure for SARS-CoV- 2. LLLT is a thriving technology used to treat different kinds of conditions that require stimulation of healing, relief of pain and inflammation, and repair of function. LLLT has been used to treat wounds, sports injuries, chronic pain, dermatitis, and hair loss. Although LLLT can treat some medical conditions, it has been observed that it can cause an increase in blood antioxidants and heat shock protein expression. There are many kinds of stress caused by laser irradiation, and how a cell reacts to them depends on the kind and severity of the insult. The aim of this study is to investigate the effect of LLLT on SARS-CoV-2-infected HEK293/ACE2 cells. In this study, biological assays, including MTT assay, cell viability live/dead, green/deep red assay, and reactive oxygen species (ROS) detection assay, were used to determine the effects of laser irradiation on SARS-CoV-2 infected and uninfected HEK293/ACE2. HEK29/ACE2 cells were infected with SARS-CoV-2 and 48 hours post-infection they were subject to laser irradiation. After 24 hours post-irradiation, biological assays were performed. Both infected non-irradiated and irradiated cells displayed signs of cell stress and produced ROS. There were more dead cells observed in infected HEK293/ACE2 cells, while more viable cells were seen in the uninfected irradiated HEK293/ACE2 cells. LLLT can be used to explore the therapeutic qualities of laser light in SARS-CoV-2 research. LLLT can be used to explore the therapeutic effects of laser light in SARS-CoV-2 research.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

L. Mngwengwe, M. Y. Lugongolo, S. Ombinda-Lemboumba, Y. Ismail, and P. Mthunzi-Kufa "The effect of low-level laser therapy on severe acute respiratory syndrome coronavirus-2 infected cells", Proc. SPIE 12826, Mechanisms of Photobiomodulation Therapy XVIII, 1282606 (12 March 2024); https://doi.org/10.1117/12.3002453

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