A 'sono-optical' microfluidic device for induced sustained rotation and manipulation of biological samples

Mia K. Løvmo; Benedikt Pressl; Gregor Thalhammer; Monika Ritsch-Marte

doi:10.1117/12.2567897

22 August 2020 A 'sono-optical' microfluidic device for induced sustained rotation and manipulation of biological samples

Mia K. Løvmo, Benedikt Pressl, Gregor Thalhammer, Monika Ritsch-Marte

Author Affiliations +

Proceedings Volume 11463, Optical Trapping and Optical Micromanipulation XVII; 114630R (2020) https://doi.org/10.1117/12.2567897
Event: SPIE Nanoscience + Engineering, 2020, Online Only

Abstract

We combine acoustic and optical trapping in a versatile, low-cost micro-fluidic chip for contact-free manipulation and imaging of sub-millimeter sized live biological samples in liquids. Our fully reconfigurable hybrid ‘sono-optical’ device opens up for 3D patterning where ultrasound in three orthogonal directions provide confinement and alignment of the sample suspended in the resonator, and tunable holographic optical tweezers enable us to modify and refine the acoustic trapping landscape on a finer spatial scale. We can induce sustained rotations of samples, as spheroids, embryos etc., providing access to the image data required for volumetric reconstruction of the sample by diffuse optical tomography. Our approach paves the way for long-term biological studies of micro-organism, developing embryos or larvae, or of cancer spheroids and organoids, in terms of local or global mechanical probing or in terms of non-invasive 3D visual inspection.

Conference Presentation

Citation Download Citation

Mia K. Løvmo, Benedikt Pressl, Gregor Thalhammer, and Monika Ritsch-Marte "A 'sono-optical' microfluidic device for induced sustained rotation and manipulation of biological samples", Proc. SPIE 11463, Optical Trapping and Optical Micromanipulation XVII, 114630R (22 August 2020); https://doi.org/10.1117/12.2567897

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