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Combining optical tweezers with acoustic trapping in one platform allows us to trap and manipulate sub-millimeter sized biological samples in suspension in a contact-less and flexible manner. The acoustic radiation forces levitate and trap the sample and steerable holographic optical tweezers give us an additional means of manipulation. We have implemented 3D acoustic trapping on a microfluidic chip, with three independent MHz transducers in three orthogonal directions; two side-transducers and one transparent top-transducer facilitating optical access for optical trapping and imaging. We can reorient the samples, or induce sustained rotations to gain access to multiple viewing angles of the object.
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Mia Kvåle Løvmo, Simon Moser, Gregor Thalhammer, Monika Ritsch-Marte, "Hybrid optical and 3D acoustic trapping in a 'sono-optical' microfluidic chip for flexible manipulation of biological samples," Proc. SPIE PC12198, Optical Trapping and Optical Micromanipulation XIX, PC121981A (3 October 2022); https://doi.org/10.1117/12.2633306