Enhanced total internal reflection fluorescence imaging based on dielectric resonant multilayers for multimodal fluorescence microscopy

Aude L. Lereu; Anita Mouttou; Fabien Le Marchand; Thomas Begou; Antonin Moreau; Delphine Muriaux; Julien Lumeau; Cyril Favard

doi:10.1117/12.2567941

20 August 2020 Enhanced total internal reflection fluorescence imaging based on dielectric resonant multilayers for multimodal fluorescence microscopy

Aude L. Lereu, Anita Mouttou, Fabien Le Marchand, Thomas Begou, Antonin Moreau, Delphine Muriaux, Julien Lumeau, Cyril Favard

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Proceedings Volume 11461, Active Photonic Platforms XII; 114610E (2020) https://doi.org/10.1117/12.2567941
Event: SPIE Nanoscience + Engineering, 2020, Online Only

Abstract

Design in a proper way, optical dielectric resonant multilayers can support huge optical field enhancement when working under total internal reflection. TIRFM - Total Internal Reflection Fluorescence Microscopy, also based on total internal reflection illumination, is used in cell biology imaging where many biological processes involve cell membranes and their immediate intracellular spatial environment (signaling, cellular traffic, adhesion...). We propose to develop an enhanced version of TIRFM by investigating the optimization, conception and implementation of dedicated optical dielectric resonant multilayers as the glass coverslip replacement. Model samples such as lipid bilayers with a known thickness will be first investigated but our ultimate goal is to image more complex biological processes such as viral budding, or molecular transport mechanisms such as exocytosis or endocytosis.

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Aude L. Lereu, Anita Mouttou, Fabien Le Marchand, Thomas Begou, Antonin Moreau, Delphine Muriaux, Julien Lumeau, and Cyril Favard "Enhanced total internal reflection fluorescence imaging based on dielectric resonant multilayers for multimodal fluorescence microscopy", Proc. SPIE 11461, Active Photonic Platforms XII, 114610E (20 August 2020); https://doi.org/10.1117/12.2567941

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KEYWORDS

Luminescence

Dielectrics

Microscopy

Adaptive optics

Image processing

Signal processing

Cell biology

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