In this work, we evaluate quantitatively the three-dimensional fluorescence imaging based transport of intensity equation (TIE). In the TIE-based fluorescence imaging, phase information from the recorded defocus fluorescence images is retrieved by solving the phase retrieval algorithm. From the retrieved phase data and their corresponding fluorescence image, the complex function can reconstructed. Focus images at different distances can be obtained after applying numerical inverse Fresnel propagation of complex function. For the quantitative evaluation of retrieved focus images, we calculate cross correlation coefficients to check intensity conservation.
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