Dr. Wanaruk Chaimayo Profile

Dr. Wanaruk Chaimayo

Postdoctoral Research Associate at Univ of Rochester Medical Ctr

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Proceedings Article | 2 March 2015 Paper

Examining small molecule: HIV RNA interactions using arrayed imaging reflectometry

Wanaruk Chaimayo, Benjamin Miller

Proceedings Volume 9310, 931006 (2015) https://doi.org/10.1117/12.2085058

KEYWORDS: Molecules, Imaging arrays, Reflectometry, Silica, Silicon, Proteins, Reflectivity, Prisms, Target detection, Molecular interactions

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Human Immunodeficiency Virus (HIV) has been the subject of intense research for more than three decades as it causes an uncurable disease: Acquired Immunodeficiency Syndrome, AIDS. In the pursuit of a medical treatment, RNAtargeted small molecules are emerging as promising targets. In order to understand the binding kinetics of small molecules and HIV RNA, association (k_a) and dissociation (k_d) kinetic constants must be obtained, ideally for a large number of sequences to assess selectivity. We have developed Aqueous Array Imaged Reflectometry (Aq-AIR) to address this challenge. Using a simple light interference phenomenon, Aq-AIR provides real-time high-throughput multiplex capabilities to detect binding of targets to surface-immobilized probes in a label-free microarray format. The second generation of Aq-AIR consisting of high-sensitivity CCD camera and 12-μL flow cell was fabricated. The system performance was assessed by real-time detection of MBNL1-(CUG)₁₀ and neomycin B - HIV RNA bindings. The results establish this second-generation Aq-AIR to be able to examine small molecules binding to RNA sequences specific to HIV.

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