Oxygen partial pressure (pO2) has been measured in large retinal vessels using oxygen-dependent quenching of phosphorescent probe lifetimes, but existing systems face resolution or speed limitations due to camera-based or two-photon imaging. We developed a multimodal scanning laser ophthalmoscopy (SLO) and visible light optical coherence tomography (VIS-OCT) device to image murine retinae with balanced speed and resolution and capture additional vascular structure and function information. Phosphorescence intensity images using Oxyphor 2P demonstrated improved resolution, revealing smaller vascular structures, and improved throughput (~200 µs/pixel). VIS-OCT produced high resolution structural volumes and demonstrated doppler capabilities with alternating artery and vein phase shifts.
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