Actinic keratosis (AK) is a common cutaneous neoplasm resulting from severe solar damage. Noninvasive diagnosis and grading of AK are important not just because of AK’s precancerous nature, but also due to the frequent presence of multiple AKs.
Here we study the clinical applicability of in vivo harmonic generation microscopy (HGM) for the grading, diagnosis, and treatment assessment of AKs. As a result, 100% agreement on diagnosis and grading outcomes between HGM and traditional histopathology were obtained. Our study indicates the superior capability of in vivo HGM for diagnosis, grading, and treatment assessment of AKs.
Label-free high-resolution visualization of Alzheimer’s disease (AD) neuropathological hallmark, amyloid β (Aβ) plaques, is one of the prime goals of neuroscience. Till today, traditional histological procedures, which rely on fixation and tedious staining of tissues, can only provide definitive confirmation of AD. However, recent studies have shown that label-free third harmonic generation (THG) microscopy, a virtual transition based technology, can provide structural information of biological tissues with subcellular 3D resolution. In this study, using a 1263 nm Cr: Forsterite laser source, we performed THG studies on 3xTg AD mice brain tissues in vitro, with a focus on contrast origin evaluation for plaques. Our THG study can clearly differentiate, with very high resolution, neuropathological hallmark of AD: Aβ plaques. Moreover, THG can also distinguish white and gray matter along with axons, and soma of brain. The origin of THG contrasts for various structures of brain including AD pathological hallmarks were verified through standard immunohistochemical staining procedures. Our preliminary study has successfully demonstrated the capability of THG in revealing AD histopathological features with sub-femtoliter resolution without the need of any exogenous staining of the tissues.
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