Ms. Rashmi D. Chaudhari Profile

Rashmi D. Chaudhari

at Indian Institute of Technology Bombay

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Publications (2)

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Proceedings Article | 16 April 2014 Paper

FITC-tagged macromolecule-based alginate microspheres for urea sensoring

Abhijeet Joshi, Rashmi Chaudhari, Rohit Srivastava

Proceedings Volume 9060, 90600P (2014) https://doi.org/10.1117/12.2044898

KEYWORDS: Urea, Biosensors, Luminescence, Kidney, Sensors, Biosensing, Scanning electron microscopy, Fluorescence spectroscopy, Biological research, Urea concentration

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Urea is an important biomarker for identification of kidney diseases. Early urea detection using a specific and sensitive technique can significantly reduce the mortality of patients. The research aims at developing fluorescence-based FITCmediated pH and urea measurement. A system containing FITC-dextran in alginate microspheres was developed using air-driven atomization. pH/Urea biosensor was characterized using optical microscopy, SEM, and CLSM. Urea biosensing studies were performed by exposing different standard solutions of pH and urea standard solutions using fluorescence spectroscopy (λex=488 nm and λem=520 nm). FITC-dextran was entrapped using an encapsulation unit and alginate microspheres were formed. The microspheres were found to be uniform and spherical in nature with sizes (50±10μ). FITC-dextran was found to be uniformly distributed in the alginate microspheres as per the CLSM scans. Urea biosensing studies indicate that a linear correlation was observed with increasing urea concentrations. The said microspheres can be used to detect changes in pH from 4-8 units owing to its linear response in this range. FITC dextran loaded alginate microspheres showed an improved range of detection upto 7 mM in comparison to 1.5 mM when in solution phase in a study with urea concentrations from 0-50 mM. The pH and urea detection was accurate to an extent of interday variation of 5%. FITC-dextran loaded alginate microspheres show a great potential for usage as a pH and urea biosensor for early detection of kidney diseases.

Proceedings Article | 16 April 2014 Paper

Oxygen sensing glucose biosensors based on alginate nano-micro systems

Rashmi Chaudhari, Abhijeet Joshi, Rohit Srivastava

Proceedings Volume 9060, 906005 (2014) https://doi.org/10.1117/12.2044894

KEYWORDS: Glucose, Oxygen, Biosensors, Biosensing, Luminescence, Blood, Sensors, Nanoparticles, Particles, Near infrared

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Clinically glucose monitoring in diabetes management is done by point-measurement. However, an accurate, continuous glucose monitoring, and minimally invasive method is desirable. The research aims at developing fluorescence-mediated glucose detecting biosensors based on near-infrared radiation (NIR) oxygen sensitive dyes. Biosensors based on Glucose oxidase (GOx)-Rudpp loaded alginate microspheres (GRAM) and GOx-Platinum-octaethylporphyrin (PtOEP)-PLAalginate microsphere system (GPAM) were developed using air-driven atomization and characterized using optical microscopy, CLSM, fluorescence spectro-photometry etc. Biosensing studies were performed by exposing standard solutions of glucose. Uniform sized GRAM and GPAM with size 50±10μm were formed using atomization. CLSM imaging of biosensors suggests that Rudpp and PtOEP nanoparticles are uniformly distributed in alginate microspheres. The GRAM and GPAM showed a good regression constant of 0.974 and of 0.9648 over a range of 0-10 mM of glucose with a high sensitivity of 3.349%/mM (625 nm) and 2.38%/mM (645 nm) at 10 mM of glucose for GRAM and GPAM biosensor. GRAM and GPAM biosensors show great potential in development of an accurate and minimally invasive glucose biosensor. NIR dye based assays can aid sensitive, minimally-invasive and interference-free detection of glucose in diabetic patients.

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