The development and optimization of photopolymers in which time-stable holographic grating can be stored when the material is immersed in an aqueous medium represent a challenge at present. In this sense, the aim of this work was the fabrication of unslanted transmission gratings in a hydrogel matrix which incorporates in its molecular structure a monomer (2-Methacryloyloxyethyl phosphorylcholine) that can act as an anti-fouling agent. The dependence of the diffraction efficiency on the thickness of the hydrogel matrix and the stability of the transmission gratings immersed in water were also studied. An increment in the diffraction efficiency up to 47 % was observed after a washing stage. After two days immersed in water, the holograms showed high stability even though the diffraction efficiency decreased to 37%. The optical parameters were obtained by fitting procedure through Kogelnik’s coupled wave theory.
In today’s point-of-care testing (POCT), there is an ever-increasing demand for novel and more efficient devices for early diagnosis, especially in cardiovascular diseases (CVD). Early detection of CVD markers, such as Troponin present in the bloodstream, is a key factor for reducing CVD mortality rates.
Thiol-ene coupling (TEC) and Light Assisted Molecular Immobilization (LAMI) are photonic techniques leading to immobilization of bioreceptors, such as, antibodies which recognize cardiac markers. These techniques present advantages compared to traditional immobilization techniques since, e.g., there are no thermal or chemical steps and they work in water media. TEC reaction takes place at close-to-visible wavelengths (λ=365nm) which induces the formation of thiol radicals which bind to alkene functional group on the surface through a thioether bond. LAMI secures molecular immobilizations in a spatially oriented, localized and covalent coupling of biomolecules onto thiol reactive surfaces down to submicrometer spatial resolution. LAMI is possible due to a conserved structural motif in proteins: the spatial proximity between aromatic residues and disulfide bridges. When aromatic residues are excited with UV light (275- 295nm), disulphide bridges are disrupted and free thiol groups are formed that can bind covalently to a surface decorated with thiol groups.
We have achieved successful immobilization of anti-troponin and anti-myoglobin antibodies with both photonic immobilization techniques. The microarrays of immobilized monoclonal antibodies have successfully detected the CVD biomarkers troponin I and myoglobin, as confirmed by fluorescence imaging. A sandwich immunoassay was carried out, Troponin I and Myoglobin were detected down to 10 ng/mL and 1 ng/mL, respectively.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.