Mr. Martin J. Sweetman Profile

Martin J. Sweetman

PhD student

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Proceedings Article | 27 December 2007 Paper

Interfacing porous silicon with biomolecules

Martin Sweetman, Sean Graney, Nicolas Voelcker

Proceedings Volume 6799, 679907 (2007) https://doi.org/10.1117/12.759211

KEYWORDS: Silicon, Proteins, Semiconducting wafers, Luminescence, Atomic force microscopy, Nitrogen, Biosensors, Infrared spectroscopy, Etching, Oxidation

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The control of protein binding into nanostructured porous surfaces is highly relevant to the development of advanced biosensors and other biodevices. Here, an investigation of the covalent immobilisation of a model protein (albumin) onto porous silicon (pSi) films was conducted using a new alkene linker, the synthesis of which was developed. This alkene linker contained both hydrophobic and hydrophilic (oligoethylene glycol) sections and terminated in a protected thiol. The alkene was attached to freshly etched porous silicon via thermal hydrosilylation, where further surface reactions resulted in the attachment of a maleimido N-hydroxysuccinimidyl (NHS) heterobifunctional crosslinker. Albumin was then covalently immobilised on the porous silicon layer through reaction of the protein's amine groups and the NHS functional group of the crosslinker. Surface modification reactions were monitored by infrared spectroscopy and interferometric reflectance spectroscopy. Protein binding was monitored by infrared spectroscopy, fluorescence imaging and atomic force microscopy.

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