Dr. Kelvin W. C. Poon Profile

Dr. Kelvin W. C. Poon

Research Analyst at University of Calgary

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Author

Publications (5)

SPIE Journal Paper | 21 April 2019 Open Access

Excitation parameters optimized for coherent anti-Stokes Raman scattering imaging of myelinated tissue

Craig Brideau, Kelvin W. Poon, Pina Colarusso, Peter Stys

JBO, Vol. 24, Issue 04, 046502, (April 2019) https://doi.org/10.1117/12.10.1117/1.JBO.24.4.046502

KEYWORDS: Polarization, Imaging systems, CARS tomography, Tissues, Raman spectroscopy, Axons, Calibration, Polymers, Polarization control, Control systems

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Proceedings Article | 10 March 2015 Paper

Quantitative biochemical investigation of various neuropathologies using high-resolution spectral CARS microscopy

Kelvin Poon, Craig Brideau, Geert Schenk, Roel Klaver, Antoine Klauser, Jean Kawasoe, Jeroen Geurts, Peter Stys

Proceedings Volume 9305, 930504 (2015) https://doi.org/10.1117/12.2076654

KEYWORDS: Microscopy, Imaging systems, Linear filtering, Sensors, Biochemistry, Mirrors, Reflectors, Digital imaging, Detection and tracking algorithms, Channel projecting optics

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The pathology of multiple sclerosis involves the gray and white matter regions of the brain and spinal cord often characterized by various combinations of demyelination, inflammatory infiltration, axonal degeneration, and later gliosis in chronic lesions. While acute and chronic white matter lesions are well characterized and easily identified, evidence indicates that the CNS of MS patients may be globally altered, with subtle abnormalities found in grossly normal appearing white matter (NAWM) with histochemical stains and magnetic resonance imaging only indicating a general alteration in tissue composition at best. Thus, the prototypical acute inflammatory lesion may merely represent the most obvious manifestation of a chronic widespread involvement of the CNS, which is difficult to examine reliably. The current study deals with the microstructure and biochemistry of demyelination, remyelination and axonal loss in various regions in post-mortem human MS brain, especially NAWM areas around more typical acute and chronic lesions. The myelin sheath, neuroglia and perivascular spaces were investigated through changes in the intrinsic molecular vibrational signatures of lipid biochemistry using a novel, label-free Coherent anti-Stokes Raman Scattering (CARS) microscope. The biochemistry of myelin lipids can be probed by detecting subtle changes to phospholipids and the intra-molecular disorder of their fatty acid acyl chains, various oxidation products and general protein contributions. NAWM regions surrounding pathological MS lesions were shown to reveal abnormalities despite morphological classifications indicating otherwise. CARS data were correlated with immunohistochemical stains and lipophilic dyes. Spectral data were analyzed using a unique non-linear algorithm, which allows quantification and classification through gated parameters and displayed through bivariate histograms. Our CARS microscopy system provides high-resolution, detailed morphological and unique biochemical information regarding CNS pathology in human MS examples and may be applicable to a broad range of other white matter centric neurological disorders.

Proceedings Article | 5 March 2015 Paper

Adaptation of commercial microscopes for advanced imaging applications

Craig Brideau, Kelvin Poon, Peter Stys

Proceedings Volume 9329, 93292U (2015) https://doi.org/10.1117/12.2079625

KEYWORDS: Optical filters, Microscopes, Lamps, Control systems, Head, Confocal microscopy, Sensors, Light sources, Imaging systems, Collimation

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Proceedings Article | 8 March 2013 Paper

Investigation of human multiple sclerosis lesions using high resolution spectrally unmixed CARS microscopy

Kelvin Poon, Craig Brideau, Wulin Teo, Geert Schenk, Roel Klaver, Antoine Klauser, Jean Kawasoe, Jeroen J. G. Geurts, Peter Stys

Proceedings Volume 8565, 85654V (2013) https://doi.org/10.1117/12.2005504

KEYWORDS: Tissues, Microscopy, Brain, Raman spectroscopy, CARS tomography, Polymers, Axons, Pathology, Luminescence, Spectral resolution

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Proceedings Article | 22 February 2013 Paper

Broadly tunable high-energy spectrally focused CARS microscopy with chemical specificity and high resolution for biological samples

C. Brideau, K. Poon, P. Stys

Proceedings Volume 8588, 85880E (2013) https://doi.org/10.1117/12.2005512

KEYWORDS: Optical parametric oscillators, Microscopy, Imaging systems, Raman spectroscopy, Fiber lasers, Picosecond phenomena, Photons, Femtosecond phenomena, Microscopes, Glasses

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