Ms. Jessica A. Ogden Profile

Jessica A. Ogden

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Publications (5)

Proceedings Article | 24 February 2009 Paper

Assessment of intratumor non-antibody directed iron oxide nanoparticle hyperthermia cancer therapy and antibody directed IONP uptake in murine and human cells

P. Hoopes, J. Tate, J. Ogden, R. Strawbridge, S. Fiering, A. Petryk, S. Cassim, A. Giustini, E. Demidenko, R. Ivkov, S. Barry, P. Chinn, A. Foreman

Proceedings Volume 7181, 71810P (2009) https://doi.org/10.1117/12.812056

KEYWORDS: Tumors, Nanoparticles, Iron, Particles, Oxides, Cancer, In vivo imaging, In vitro testing, Tissues, Oncology

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Hyperthermia, as an independent modality or in combination with standard cancer treatments such as chemotherapy and radiation, has been established in vitro and in vivo as an effective cancer treatment. However, despite efforts over the past 25 years, such therapies have never been optimized or widelyaccepted clinically. Although methods continue to improve, conventionally-delivered heat (RF, ultrasound, microwave etc) can not be delivered in a tumor selective manner. The development of antibody-targeted, or even nontargeted, biocompatible iron oxide nanoparticles (IONP) now allows delivery of cytotoxic heat to individual cancer cells. Using a murine mouse mammary adenocarcinoma (MTGB) and human colon carcinoma (HT29) cells, we studied the biology and treatment of IONP hyperthermia tumor treatment. Methods: Cancer cells (1 x 10⁶) with or without iron oxide nanoparticles (IONP) were studied in culture or in vivo via implanted subcutaneously in female C3H mice, Tumors were grown to a treatment size of 150 mm³ and tumors volumes were measured using standard 3-D caliper measurement techniques. Mouse tumors were heated via delivery of an alternating magnetic field, which activated the nanoparticles, using a cooled 36 mm diameter square copper tube induction coil which provided optimal heating in 1.5 cm wide region of the coil. The IONPs were dextran coated and had a hydrodynamic radius of approximately 100 nm. For the in vivo studies, intra-tumor, peritumor and rectal (core body) temperatures were continually measured throughout the treatment period. Results: Although some eddy current heating was generated in non-target tissues at the higher field strengths, our preliminary IONP hyperthermia studies show that whole mouse AMF exposure @160 KHz and 400 or 550 Oe, for a 20 minutes (heat-up and protocol heating), provides a safe and efficacious tumor treatment. Initial electron and light microscopic studies (in vitro and in vivo) showed the 100 nm used in our studies are rapidly taken up and retained by the tumor cells. Additional in vitro studies suggest antibodies can significantly enhance the cellular uptake of IONPs.

Proceedings Article | 12 February 2009 Paper

Comparison of iron oxide nanoparticle and waterbath hyperthermia cytotoxicity

J. Ogden, J. Tate, R. Strawbridge, R. Ivkov, P. Hoopes

Proceedings Volume 7181, 71810K (2009) https://doi.org/10.1117/12.809818

KEYWORDS: Particles, Magnetism, Transmission electron microscopy, Cancer, Iron, Oxides, Nanoparticles, Cell death, Tissues, Temperature metrology

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Proceedings Article | 12 February 2009 Paper

Toxicity and biodistribution of activated and non-activated intravenous iron oxide nanoparticles

J. Tate, J. Ogden, R. Strawbridge, Z. Pierce, P. Hoopes

Proceedings Volume 7181, 71810L (2009) https://doi.org/10.1117/12.809830

KEYWORDS: Iron, Magnesium, Liver, Blood, Nanoparticles, Kidney, Particles, Proteins, Spleen, Toxicity

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Proceedings Article | 13 February 2007 Paper

Intratumoral iron oxide nanoparticle hyperthermia and radiation cancer treatment

P. J. Hoopes, R. Strawbridge, U. Gibson, Q. Zeng, Z. Pierce, M. Savellano, J. Tate, J. Ogden, I. Baker, R. Ivkov, A. Foreman

Proceedings Volume 6440, 64400K (2007) https://doi.org/10.1117/12.706302

KEYWORDS: Tumors, Nanoparticles, Iron, Oxides, Particles, Cancer, Oncology, Tissues, Temperature metrology, Breast cancer

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The potential synergism and benefit of combined hyperthermia and radiation for cancer treatment is well established, but has yet to be optimized clinically. Specifically, the delivery of heat via external arrays /applicators or interstitial antennas has not demonstrated the spatial precision or specificity necessary to achieve appropriate a highly positive therapeutic ratio. Recently, antibody directed and possibly even non-antibody directed iron oxide nanoparticle hyperthermia has shown significant promise as a tumor treatment modality. Our studies are designed to determine the effects (safety and efficacy) of iron oxide nanoparticle hyperthermia and external beam radiation in a murine breast cancer model. Methods: MTG-B murine breast cancer cells (1 x 106) were implanted subcutaneous in 7 week-old female C3H/HeJ mice and grown to a treatment size of 150 mm3 +/- 50 mm3. Tumors were then injected locally with iron oxide nanoparticles and heated via an alternating magnetic field (AMF) generator operated at approximately 160 kHz and 400 - 550 Oe. Tumor growth was monitored daily using standard 3-D caliper measurement technique and formula. specific Mouse tumors were heated using a cooled, 36 mm diameter square copper tube induction coil which provided optimal heating in a 1 cm wide region in the center of the coil. Double dextran coated 80 nm iron oxide nanoparticles (Triton Biosystems) were used in all studies. Intra-tumor, peri-tumor and rectal (core body) temperatures were continually measured throughout the treatment period. Results: Preliminary in vivo nanoparticle-AMF hyperthermia (167 KHz and 400 or 550 Oe) studies demonstrated dose responsive cytotoxicity which enhanced the effects of external beam radiation. AMF associated eddy currents resulted in nonspecific temperature increases in exposed tissues which did not contain nanoparticles, however these effects were minor and not injurious to the mice. These studies also suggest that iron oxide nanoparticle hyperthermia is more effective than non-nanoparticle tumor heating techniques when similar thermal doses are applied. Initial electron and light microscopy studies of iron oxide nanoparticle and AMF exposed tumor cells show a rapid uptake of particles and acute cytotoxicity following AMF exposure.

Proceedings Article | 9 February 2007 Paper

In-vitro investigations of nanoparticle magnetic thermotherapy: adjuvant effects and comparison to conventional heating

Z. Pierce, R. Strawbridge, C. Gaito, L. Dulatas, J. Tate, J. Ogden, P. J. Hoopes

Proceedings Volume 6440, 64400J (2007) https://doi.org/10.1117/12.710579

KEYWORDS: Nanoparticles, Magnetism, Heat therapy, Iron, Oxides, Particles, In vitro testing, Radiotherapy, Temperature metrology, Cancer

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