We built a fibre optic illuminated deep ultraviolet (DUV) fluorescence microscope. Our microscope was designed to be compact and portable, sitting on a 30cm by 30cm breadboard. Multimode fibres were magnetically butt coupled to 2 DUV light emitting diodes, which provided oblique epi-illumination of biological samples with short working distance objectives. The stage was motorised by stepper motors and controlled by a microcontroller which allowed for high-resolution imaging of large, fresh, and unsectioned tissue with high-throughput. We demonstrated the fluorescence microscope across a wide range of biological samples from single cells and spheroids to large tumours and organ samples.
High-resolution images are necessary for various biological, clinical, and pathological applications. However, using objective lenses with high magnifications comes with its limitations: a smaller field of view, shorter working distance, limited depth of field, more intense illumination required, and a more complex illumination setup required. Single image super-resolution (SISR) could allow us to generate high-resolution images while still reaping the benefits of using low-magnification objectives.
We developed SRFlow++, a conditional normalizing flow for SISR to empower our microscope with the ability to produce high-resolution (10x magnified) images of high fidelity and quality while using a low-magnification (4x) objective.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.