Prof. Jeffrey R. Morgan Profile

Prof. Jeffrey R. Morgan

at Brown Univ

SPIE Involvement:

Author

Publications (2)

SPIE Journal Paper | 29 October 2024 Open Access

Mueller matrix analysis of a biologically sourced engineered tissue construct as polarimetric phantom

Zixi Lin, Samantha Madnick, Joshua Burrow, Jeffrey Morgan, Kimani Toussaint

JBO, Vol. 29, Issue 10, 106002, (October 2024) https://doi.org/10.1117/12.10.1117/1.JBO.29.10.106002

KEYWORDS: Tissues, Polarimetry, Collagen, Depolarization, Mueller matrices, Second harmonic generation, Biological samples, Biological research, Polarization, Biological imaging

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SignificanceThe polarimetric properties of biological tissues are often difficult to ascertain independent of their complex structural and organizational features. Conventional polarimetric tissue phantoms have well-characterized optical properties but are overly simplified. We demonstrate that an innovative, biologically sourced, engineered tissue construct better recapitulates the desired structural and polarimetric properties of native collagenous tissues, with the added benefit of potential tunability of the polarimetric response. We bridge the gap between non-biological polarimetric phantoms and native tissues.AimWe aim to evaluate a synthesized tissue construct for its effectiveness as a phantom that mimics the polarimetric properties in typical collagenous tissues.ApproachWe use a fibroblast-derived, ring-shaped engineered tissue construct as an innovative tissue phantom for polarimetric imaging. We perform polarimetry measurements and subsequent analysis using the Mueller matrix decomposition and Mueller matrix transformation methods. Scalar polarimetric parameters of the engineered tissue are analyzed at different time points for both a control group and for those treated with the transforming growth factor (TGF)-β1. Second-harmonic generation (SHG) imaging and three-dimensional collagen fiber organization analysis are also applied.ResultsWe identify linear retardance and circular depolarization as the parameters that are most sensitive to the tissue culture time and the addition of TGF-β1. Aside from a statistically significant increase over time, the behavior of linear retardance and circular depolarization indicates that the addition of TGF-β1 accelerates the growth of the engineered tissue, which is consistent with expectations. We also find through SHG images that collagen fiber organization becomes more aligned over time but is not susceptible to the addition of TGF-β1.ConclusionsThe engineered tissue construct exhibits changes in polarimetric properties, especially linear retardance and circular depolarization, over culture time and under TGF-β1 treatments. This tissue construct has the potential to act as a controlled modular optical phantom for polarimetric-based methods.

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Proceedings Article | 13 March 2019 Presentation

Label-free cellular viability imaging in 3D tissue spheroids with dynamic optical coherence tomography (Conference Presentation)

Ahbid Zein-Sabatto, Julia Lee, Madison Kuhn, Itzel Aponte, Bruno Felalaga, Jessica Sevetson, Blanche Ip, Diane Hoffman-Kim, Jeffrey Morgan, Jonghwan Lee

Proceedings Volume 10883, 108830T (2019) https://doi.org/10.1117/12.2508845

KEYWORDS: Optical coherence tomography, 3D image processing, Stereoscopy, Tissue optics, 3D metrology, Luminescence, Diffusion, 3D displays, Tissues, Cancer

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