KEYWORDS: 3D modeling, Optical coherence tomography, Imaging systems, Tumors, 3D image processing, Bessel beams, 3D metrology, 3D acquisition, Visualization, Tissues
3D cell models indicate stronger similarities in vivo than monolayer cell culture, and thus is raising awareness as an important tool for evaluating biological phenomena, drug action, and mechanism of diseases. This paper compared two imaging systems for accurate measurements of the morphological structure and volume quantification of 3D tumor spheroids. The two imaging systems are OCT using a scan lens and EDOF-OCM, which uses a Bessel beam to expand the depth of field. Human hepatoma cells were used for spheroid formation. To compare the effectiveness of the imaging system, we measured the spheroid structure according to cell number per spheroid and growth per day. Although both systems were able to acquire the morphological structure and quantify volume of 3D tumor spheroids the OCM using the Bessel beam was able to more accurately measure the overall structure of the spheroid without surface reflection.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.