We present an endoscopic system allowing to perform 3-photon excitation fluorescence and third harmonic generation imaging. The ultrashort pulses required for multiphoton excitation are delivered from an ultrafast laser system to the endoscopic probe using a connectorized hollow core delivery fiber.
We demonstrate a highly multimodal nonlinear micro-endoscope for real-time, label-free imaging of biological tissues. The endoscope can perform two and three photon excited fluorescence, second, third harmonic and CARS imaging for different excitation wavelengths. Ultrashort pulses are delivered to the sample by a double-clad antiresonant hollow core fiber over the 800-1800 nm spectral band. The fiber tip is placed into a doubly resonant piezoelectric tube which allows a spiral scanning on the sample. The endoscope distal head containing the scanning device and the GRIN micro-objective is 1.5 mm in diameter and 35 mm long. Real-time nonlinear imaging at 10 frame/s is demonstrated.
We demonstrate 3-photon fluorescence micro-endoscopy using a negative curvature hollow core fiber and a 2.2 mm miniature scanning head. The fiber design allows distortion-less, delivery of <100 fs pulses without dispersion pre-compensation requirements. The fiber also features a double cladding allowing the back-collection of nonlinear signals through the same fiber. Sub-micron spatial resolution together with large field of view is made possible by the combination of a miniature distal objective lens with a functionnalization of the fiber output with a GRIN fiber spliced to the output facet. 3-photon fluoresence imaging is demonstrated on various biological samples.
We present a flexible, compact, 2photon, 3photon, SHG and CARS nonlinear endo-microscope featuring a 2.2 mm outer diameter and a length of 34 mm. It uses a negative curvature hollow-core double-clad fiber that is scanned with a resonant piezo-scanner. The fiber design allows distortion-less, background-free delivery of femtosecond and picosecond excitation pulses and the back-collection of nonlinear signals through the same fiber. Sub-micron spatial resolution together with >300 microns field of view is made possible using micro-lenses or GRIN based miniature objective lens. We demonstrate 2-photon and 3-photon fluorescence, SHG, THG and CARS imaging at a rate of 10 frames/s.
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