Mr. Daniel Kraus Profile

Daniel Kraus

at Univ of the Sciences in Philadelphia

SPIE Involvement:

Author

Publications (3)

SPIE Journal Paper | 20 September 2021 Open Access

Small molecule kinase inhibitors enhance aminolevulinic acid-mediated protoporphyrin IX fluorescence and PDT response in triple negative breast cancer cell lines

Pratheeba Palasuberniam, Daniel Kraus, Matthew Mansi, Richard Howley, Alexander Braun, Kenneth Myers, Bin Chen

JBO, Vol. 26, Issue 09, 098002, (September 2021) https://doi.org/10.1117/12.10.1117/1.JBO.26.9.098002

KEYWORDS: Luminescence, Photodynamic therapy, Tumors, Molecules, Cell death, Confocal microscopy, Breast cancer, Flow cytometry, Breast, Surgery

Read Abstract +

Significance: We demonstrate that clinically used kinase inhibitors such as lapatinib can be used for enhancing aminolevulinic acid (ALA) for tumor fluorescence imaging and photodynamic therapy (PDT). Aim: ALA is used as a prodrug for protoporphyrin IX (PpIX) fluorescence-guided tumor resection and PDT. Our previous studies indicate that tumors with high ABCG2 activity exhibit low PpIX fluorescence, which hampers the application of ALA. We aim to determine whether clinically used ABCG2-interacting kinase inhibitors increase ALA-PpIX fluorescence and PDT. Approach: PpIX fluorescence was determined by spectrofluorometry, flow cytometry, and confocal microscopy after ALA alone or in combination with kinase inhibitors in triple negative breast cancer (TNBC) cell lines. Cytotoxicity was examined after ALA-PDT alone or in combination with kinase inhibitors. Effect of single and combination treatments on apoptosis was assessed by Western blot. Results: Four kinase inhibitors (lapatinib, PD169316, sunitinib, gefitinib) significantly increased ALA-PpIX fluorescence and PDT response in TNBC cells with ABCG2 activity, but not in MCF10A nontumor breast epithelial cell line without ABCG2 activity. Confocal microscopic imaging showed that PpIX fluorescence was weak and diffuse after ALA alone, which was greatly enhanced by kinase inhibitors, particularly in the mitochondria. Lapatinib was the only inhibitor that significantly reduced PpIX efflux in cell culture medium and showed stronger enhancement of PDT response than other kinase inhibitors. Lapatinib, in combination with ALA, induced tumor cells to undergo apoptosis, whereas no apoptosis was detected after each individual treatment. Conclusions: Although all four kinase inhibitors were able to enhance ALA-PpIX fluorescence and PDT, lapatinib exhibited the strongest enhancement effect. As an FDA-approved kinase inhibitor for breast cancer treatment, lapatinib is ready to be used in combination with ALA for therapeutic enhancement in tumors with elevated ABCG2 activity. This rational combination approach warrants further investigation in tumor models.

DOWNLOAD PAPER

Proceedings Article | 1 March 2016 Presentation + Paper

Combination of verteporfin-PDT and PI3K inhibitors enhances cell growth inhibition and apoptosis in endothelial cells

Daniel Kraus, Bin Chen

Proceedings Volume 9694, 969407 (2016) https://doi.org/10.1117/12.2213419

KEYWORDS: Photodynamic therapy, Cell death, Cancer, Oncology, Injuries, Oxygen, Clinical trials, Drug development, Tumors, Neodymium, Colorectal cancer, Prostate cancer, Ferroelectric polymers

Read Abstract +

Proceedings Article | 2 March 2015 Paper

Effects of verteporfin-mediated photodynamic therapy on endothelial cells

Daniel Kraus, Bin Chen

Proceedings Volume 9308, 93080K (2015) https://doi.org/10.1117/12.2080823

KEYWORDS: Photodynamic therapy, Proteins, Cell death, Luminescence, Oxygen, Confocal microscopy, Molecules, Cancer, Statistical analysis, Microscopes

Read Abstract +

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Keywords/Phrases

Search In:

Publication Years