Prof. Chung-Ren Chris Wang Profile

Prof. Chung-Ren Chris Wang

at National Chung Cheng Univ

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Publications (7)

SPIE Journal Paper | 7 May 2012 Open Access

Gold-nanorod contrast-enhanced photoacoustic micro-imaging of focused-ultrasound induced blood-brain-barrier opening in a rat model

Po-Hsun Wang, Meng-Lin Li, Hao-Li Liu, Po-Hung Hsu, Chia-Yu Lin, Chung-Ren Wang, Pin-Yuan Chen, Kuo-Chen Wei, Tzu-Chen Yen

JBO, Vol. 17, Issue 6, 061222, (May 2012) https://doi.org/10.1117/12.10.1117/1.JBO.17.6.061222

KEYWORDS: Blood brain barrier, Brain, Photoacoustic spectroscopy, Absorption, Blood, Neuroimaging, Magnetic resonance imaging, Animal model studies, Gold, Ultrasonography

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Proceedings Article | 23 February 2011 Paper

Thermotherapy with a photoacoustic/ultrasound dual-modality agent

Yu-Hsin Wang, Ai-Ho Liao, Jui-Hao Chen, Yi-Hsien Lee, Churng-Ren Wang, Pai-Chi Li

Proceedings Volume 7899, 78993V (2011) https://doi.org/10.1117/12.875667

KEYWORDS: Heat therapy, Continuous wave operation, Ultrasonography, Absorption, Gold, Spectroscopy, Photoacoustic spectroscopy, Signal to noise ratio, Temperature metrology, Nanoparticles

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Proceedings Article | 24 February 2010 Paper

Photoacoustic micro-imaging of focused ultrasound induced blood-brain-barrier opening in a rat model

Po-Hsun Wang, Po-Hung Hsu, Hao-Li Liu, Churng-Ren Chris Wang, Meng-Lin Li

Proceedings Volume 7564, 75642U (2010) https://doi.org/10.1117/12.841811

KEYWORDS: Blood brain barrier, Brain, Ultrasonography, Absorption, Photoacoustic spectroscopy, In vivo imaging, Neuroimaging, Tissue optics, Photoacoustic microscopy, Tissues

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Proceedings Article | 24 February 2010 Paper

Design and synthesis of new gold nanoparticles for enhanced photoacoustic response

Chen-Wei Wei, Carolina Poe, Chi-Meng Chen, Yi-Hsien Lee, Churng-Ren Chris Wang, Pai-Chi Li

Proceedings Volume 7564, 75641S (2010) https://doi.org/10.1117/12.842796

KEYWORDS: Photoacoustic spectroscopy, Gold, Nanoparticles, Particles, Silica, Laser irradiation, Absorption, Nanostructures, Coating, Nanorods

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Gold nanoparticles have been used as contrast agent in photoacoustic imaging to increase the detection sensitivity. For example, gold nanorods (AuNRs) have been used in time-intensity based flow estimation and used as nanoprobes to target cancer cells for early diagnosis and effective treatment. In this study, we aimed at the design and synthesis of a new type of gold nanoparticles with enhanced photoacoustic response. The key hypothesis is to create a nanostructure that allows anisotropic heat release. Specifically, such a structure results in higher heat flux transmitting outwards from the ends of the particle and therefore a greater temperature gradient can be created. To achieve this, a layer of SiO₂ was coated along the longer axis of the gold nanorods, leaving both ends uncovered. These new particles are labeled as AuNR@nu-SiO₂ with non-uniform ("nu") coating of silica. Experiments were performed to demonstrate the enhanced photoacoustic response from AuNR@nu-SiO₂. The optical illumination was delivered by a Ti: Sapphire laser pumped by an Nd:YAG laser. A home-made photoacoustic transducer with a center frequency of 20 MHz detected the resulted acoustic signal. First, new types of particles coated with and without SiO₂ were compared to bare AuNR in order to show the ability of the new nanostructure to enhance photoacoustic response. Second, the shape stability of the new particles was evaluated by measuring the photoacoustic responses versus time after high power laser irradiation. Third, the effect of thickness of SiO₂ of AuNR@nu-SiO₂ ranges from 1 nm to 15 nm was also evaluated. Results show that the mean intensity in photoacoustic image increase about 5 dB for AuNR@nu-SiO₂ compared to bare AuNR. Also, it reveals that the normalized intensity for AuNR drops to below 0.6 while it is mostly larger than 0.7 in the case of AuNR@nu-SiO₂ under pulse laser irradiation. In other words, the new type of nanoparticles is less susceptible to shape transformation. Moreover, it is indicated that the photoaocustic response increases slightly with the thickness of SiO₂ and approach to an maximum response at 9 nm thickness. In short, these new particles can be used to achieve the same level of photoacoustic response with a fewer amount of particles, which means that there is less toxicity.

Proceedings Article | 11 March 2008 Paper

In vivo photoacoustic imaging with multiple selective targeting using bioconjugated gold nanorods

Chen-Wei Wei, Chao-Kang Liao, Ying-Yi Chen, Churng-Ren Chris Wang, Ann-Ann Ding, Dar-Bin Shiehd, Pai-Chi Li

Proceedings Volume 6856, 68560J (2008) https://doi.org/10.1117/12.764454

KEYWORDS: Gold, Tumors, Cancer, Nanoprobes, In vivo imaging, Absorption, Photoacoustic imaging, Target detection, Photoacoustic spectroscopy, Nanorods

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Proceedings Article | 6 March 2006 Paper

Single-energy wash-in photoacoustic blood flow measurements using shape transitions of gold nanorods

Chen-Wei Wei, Sheng-Wen Huang, Ya-Chin Chiou, Cheng-Dah Chen, Churng-Ren Chris Wang, Pai-Chi Li

Proceedings Volume 6086, 60860L (2006) https://doi.org/10.1117/12.645272

KEYWORDS: Gold, Toxic industrial chemicals, Absorption, Photoacoustic spectroscopy, Ultrasonics, Laser irradiation, Transducers, Blood circulation, Acoustics, Nanorods

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Proceedings Article | 6 March 2006 Paper

Multiple targeting in photoacoustic imaging using bioconjugated gold nanorods

Pai-Chi Li, Chen-Wei Wei, Chao-Kang Liao, Cheng-Dah Chen, Kuei-Chen Pao, Churng-Ren Chris Wang, Ya-Na Wu, Dar-Bin Shieh

Proceedings Volume 6086, 60860M (2006) https://doi.org/10.1117/12.645248

KEYWORDS: Gold, Absorption, Nanoparticles, Proteins, Cancer, Photoacoustic spectroscopy, Molecules, Nanoprobes, Photoacoustic imaging, Nanorods

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Cancer cells presented altered surface molecules to encourage their growth and metastasis. Expression of oncogeneic surface molecules also play important roles in the prediction of clinical outcome and treatment response of anti-cancer drugs. It is thus conceivable that imaging of cancer lesions while simultaneously obtaining their pathogenic information at molecular level of as many oncogenic proteins as possible is of great clinical significance. Gold nanoparticles have been used as a contrast agent for photoacoustic imaging. In addition, gold nanoparticles can be bioconjugated to probe certain molecular processes. An intriguing property of gold nanoparticles is its ability to tailor its optical properties. For example, size effects on the surface plasmon absorption of spherical gold nanoparticles have shown that the peak optical absorption red-shifts with the increasing particle size. In addition, the optical absorption spectrum of cylindrical gold nanoparticles (i.e., gold nanorods) exhibits a strong absorption band that is directly related to the aspect ratio. With these unique characteristics, selective targeting can be achieved in photoacoustic molecular imaging. Specifically, gold nanorods with different aspect ratios can be bioconjugated to different antibodies. Multiple targeting and simultaneous detection can then be achieved by using laser irradiation at the respective peak optical absorption wavelength. In this study, photoacoustic multiple targeting using gold nanorods is experimentally demonstrated. We have chosen Her2 and CXCR4 as our primary target molecule as Her2 expression is associated with growth characteristics and sensitivity to Herceptin chemotherapy. On the other hand, CXCR4 expression predict the organ-specific metastatic potential of the cancer cells for clinical intervention in advance. Monoclonal antibody (mAb) against Her2/neu was conjugated to nanorods with several different aspect ratios. The agarose gel is suitable for photoacoustic signal acquisition. A wavelength tunable Ti-Sapphire laser was used for laser irradiation and a 1 MHz ultrasound transducer was used for acoustic detection. The optical wavelength of the laser was tuned between 800 nm and 940 nm, corresponding to gold nanorods of an aspect ratio ranging from 3.7 to 5.9. The results clearly show the potential of photoacoustic molecular imaging with multiple targeting in revealing different oncogene expression levels of the cancer cells.

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