The development of a real-time, non-invasive method for assessing hydration status is urgently needed in many communities such as the military and high-performance athletes. A probe-based spatially offset Raman spectroscopy system has been developed to accomplish this goal. Raman spectra, including the fingerprint and high wavenumber regions, have been collected from various tissue depths and superficial anatomical locations from collegiate athletes undergoing strength and conditioning regimens. Urine was collected alongside spectroscopy measurements to compare spectral information to urine specific gravity, a clinically relevant hydration metric. Water dynamics shown in spectral features are used to quantify hydration change during exercise regimens.
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