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1 March 2019 Dynamic visualization of the whole process of cytotoxic T lymphocytes killing B16 tumor cells in vitro
Shuhong Qi, Hua Shi, Lei Liu, Lili Zhou, Zhihong Zhang
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Journal of Biomedical Optics, Vol. 24, Issue 5, 051413 (March 2019). https://doi.org/10.1117/1.JBO.24.5.051413
Abstract
Cytotoxic T lymphocytes (CTLs) play a key role in adoptive cell therapy (ACT) by destroying tumor cells. Although some mechanisms of CTLs killing tumor cells have already been revealed, the precise dynamic information of CTLs’ interaction with tumor cells is still not known. Here, we used confocal microscopy to visualize the whole process of how CTLs kill tumor cells in vitro. According to imaging data, CTLs destroyed the target tumor cells rapidly and efficiently. Several CTLs surrounded one or more tumor cells, and the average time for CTLs destroying one or more tumor cells in vitro is dozens of minutes only. Our study displayed the temporal events of CTLs’ interaction with tumor cells at the beginning up to the point of killing them. Furthermore, the imaging data presented strong cytotoxicity of CTLs toward the specific tumor cells. These results could help us to well understand the mechanism of CTLs’ elimination of tumor cells and improve the efficacy of ACT in cancer immunotherapy.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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Shuhong Qi, Hua Shi, Lei Liu, Lili Zhou, and Zhihong Zhang "Dynamic visualization of the whole process of cytotoxic T lymphocytes killing B16 tumor cells in vitro," Journal of Biomedical Optics 24(5), 051413 (1 March 2019). https://doi.org/10.1117/1.JBO.24.5.051413
Received: 19 September 2018; Accepted: 14 February 2019; Published: 1 March 2019
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Cited by 8 scholarly publications.
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KEYWORDS
Tumors
In vitro testing
Visualization
Confocal microscopy
Signal detection
Cancer
Target recognition
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