Handheld tunable focus confocal microscope utilizing a double-clad fiber coupler for in vivo imaging of oral epithelium

Cory Olsovsky; Taylor Hinsdale; Rodrigo Cuenca; Yi-Shing Lisa Cheng; John M. Wright; Terry D. Rees; Javier A. Jo; Kristen C. Maitland

doi:10.1117/1.JBO.22.5.056008

25 May 2017 Handheld tunable focus confocal microscope utilizing a double-clad fiber coupler for in vivo imaging of oral epithelium

Cory Olsovsky, Taylor Hinsdale, Rodrigo Cuenca, Yi-Shing Lisa Cheng, John M. Wright, Terry D. Rees, Javier A. Jo, Kristen C. Maitland

Author Affiliations +

Journal of Biomedical Optics, Vol. 22, Issue 5, 056008 (May 2017). https://doi.org/10.1117/1.JBO.22.5.056008

Abstract

A reflectance confocal endomicroscope with double-clad fiber coupler and electrically tunable focus lens is applied to imaging of the oral mucosa. The instrument is designed to be lightweight and robust for clinical use. The tunable lens allows axial scanning through >250 μm in the epithelium when the probe tip is placed in contact with tissue. Images are acquired at 6.6 frames per second with a field of view diameter up to 850 μm. In vivo imaging of a wide range of normal sites in the oral cavity demonstrates the accessibility of the handheld probe. In vivo imaging of clinical lesions diagnosed as inflammation and dysplasia illustrates the ability of reflectance confocal endomicroscopy to image cellular changes associated with pathology.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Cory Olsovsky, Taylor Hinsdale, Rodrigo Cuenca, Yi-Shing Lisa Cheng, John M. Wright, Terry D. Rees, Javier A. Jo, and Kristen C. Maitland "Handheld tunable focus confocal microscope utilizing a double-clad fiber coupler for in vivo imaging of oral epithelium," Journal of Biomedical Optics 22(5), 056008 (25 May 2017). https://doi.org/10.1117/1.JBO.22.5.056008

Received: 16 December 2016; Accepted: 8 May 2017; Published: 25 May 2017

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