High-speed phase-shifting common-path quantitative phase imaging with a piezoelectric actuator

Séverine Coquoz; Amir Nahas; Miguel Sison; Antonio Lopez; Theo Lasser

doi:10.1117/1.JBO.21.12.126019

23 December 2016 High-speed phase-shifting common-path quantitative phase imaging with a piezoelectric actuator

Séverine Coquoz, Amir Nahas, Miguel Sison, Antonio Lopez, Theo Lasser

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Journal of Biomedical Optics, Vol. 21, Issue 12, 126019 (December 2016). https://doi.org/10.1117/1.JBO.21.12.126019

Abstract

We present a phase-shifting quantitative phase imaging technique providing high temporal and spatial phase stability and high acquisition speed. A piezoelectric microfabricated phase modulator allows tunable modulation frequencies up to the kHz range. After assessing the quantitative phase accuracy with technical samples, we demonstrate the high acquisition rate while monitoring cellular processes at temporal scales ranging from milliseconds to hours.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Séverine Coquoz, Amir Nahas, Miguel Sison, Antonio Lopez, and Theo Lasser "High-speed phase-shifting common-path quantitative phase imaging with a piezoelectric actuator," Journal of Biomedical Optics 21(12), 126019 (23 December 2016). https://doi.org/10.1117/1.JBO.21.12.126019

Received: 18 October 2016; Accepted: 5 December 2016; Published: 23 December 2016

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