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1.IntroductionAge-related macular degeneration (AMD) is a leading cause of irreversible vision loss among older adults in developed world.1 Loss of the RPE melanin was reported to be associated with AMD.2–5 Melanin in the RPE absorbs most of the light passing through the pupil and helps scavenge free radicals,1,6 which protect macular region from oxidative stress.7,8 Therefore, developing an in vivo imaging method that can quantify the RPE melanin can be clinically significant. In the past decades, researchers explored a number of methods to quantify RPE melanin.6,7,9–16 The spatial and temporal variations of RPE melanin concentration were successfully obtained by in vitro measurement. As the most direct approach, melanin concentration was roughly estimated by counting melanin granules from high-magnification micrograph9 or by counting the number of imaged pixels after Fontana-Masson staining.12,17 Later on, more accurate methods were developed. In electron spin resonance spectroscopy, spectrum of either ocular cell extracts or purified melanosomes was compared with the spectrum of synthetic DOPA melanin to determine the absolute melanin concentration.7,8 Optical absorption measurement was also reported to be employed after solubilization of free melanin granules in vitro.6,10,11 An indirect way to quantify melanin was to use high-performance liquid chromatography to measure the chemical reaction products of melanin and retrieve melanin concentration based on stoichiometric relationship.13,17 However, all the above in vitro methods required invasive procedures on the RPE sample, which made them unsuitable for clinical application. In addition, the reported methods lacked consistency among themselves, possibly resulting from variations induced by inevitable extensive chemical or biological procedures. In vitro tissue fixation also changed the tissue properties, making melanin concentration measurement challenging. To examine the RPE melanin concentration in vivo, fundus spectral analysis and near-infrared autofluorescence (NIR-AF) imaging were proposed.15,16,18 The spectrum of fundus optical reflectance was used to calculate melanin optical density based on an optical model of foveal reflection.16 However, its results were not consistent with physiological values due to oversimplified modeling of retinal tissue anatomy, optical scattering path lengths, and lack of depth resolution.19 The NIR-AF imaging has been suggested as an alternative to visualize ocular melanin because of the coincidence of strong AF signal and high melanin concentration in ocular tissues when excited by NIR light. However, a rigorous relationship between AF and melanin is still missing, and the AF signals from RPE and choroid are mixed due to the absence of depth resolution.18 A more reliable in vivo RPE melanin quantification method is still needed. Photoacoustic imaging (PAI) is an emerging technology that can directly measure optical absorption properties in tissue.20 The PA signal induced by illumination light is directly proportional to local optical energy deposition, which is related to tissue’s optical absorption coefficient.21 The optical absorption coefficient, in turn, is linearly related to pigment concentration.6,22 The ability of PAI to quantify endogenous chromophores has been investigated and confirmed by different research groups.23–25 Relative concentrations of both deoxy- and oxy-hemoglobin in blood vessels can be measured by PAI and be used to generate oxygen saturation map;23,25 PA method was also used to determine epidermal melanin content.24 Photoacoustic ophthalmoscopy (PAOM) was recently developed for retinal imaging and was able to simultaneously visualize the structure and oxygen saturation of retinal blood vessels as well as RPE melanin distribution in vivo.21,26–28 Hence, PAOM has the potential to fill the technical void of quantifying RPE melanin in vivo. However, since external factors including variation of light source intensity, placement of transducer, and orientation of eye ball may affect the absolute detected PA signal,21 longitudinal monitoring of RPE melanin is challenging. A potential solution is to use PA signals from nearby blood vessels as a reference to the PA signals from the RPE. Because hemoglobin has well-defined optical absorption properties and patients’ blood parameters, such as total hemoglobin concentration, are often easily accessible from routine blood examination, PA amplitudes from nearby vessels can be treated as an intrinsic reference to calibrate PA melanin measurements. In this work, we applied Monte Carlo (MC) simulation of photon transport in the retina to test the feasibility of the aforementioned calibrated RPE melanin quantification using PAOM. First, we examined the optical energy deposition distribution across all retinal layers in an eye model. While it has long been suggested that the majority of light is absorbed by melanin in the RPE and choroid, yet no quantitative light absorption distribution model has been proposed. A finer retinal layers segmentation model was used, for the first time, to better mimic the ocular chromophores distribution. Second, we examined the influence of PAOM axial resolution on the RPE melanin quantification. The RPE melanin together with choroidal melanin and choroidal blood are the main light absorbers in the posterior eye. Since melanin in the choroid does not vary much in life span and carries less known health value,6 only the RPE melanin is currently of clinical interest. However, the 2 layers are closely adjacent to each other and the RPE thickness is only around .29 The accuracy of measurement depends on the resolving power of PAOM along the axial direction, which is mainly determined by the ultrasonic transducer bandwidth.20 We therefore investigated the influence of PAOM axial resolution on the RPE melanin quantification. Third, we examined the RPE melanin concentration calibration scheme. The detected absolute PA signal intensity depends on several parameters including local optical absorption, excitation laser intensity, and PA detection sensitivity. In order to accurately measure the local optical absorption, the variations of laser intensity and PA detection sensitivity should be compensated for. While laser intensity can be monitored in real time, PA detection sensitivity cannot be perfectly controlled across different experiments, since PAOM relies on optical scanning.21 For accurate in vivo melanin quantification, a calibration scheme must be developed. We proposed to use retinal blood measurement to calibrate melanin measurement and further determine RPE melanin concentration. Isosbestic wavelengths for deoxy- and oxy-hemoglobin rule out the effects from variation of oxygen saturation level. Therefore, we selected the 570-nm and 584-nm as the excitation wavelengths. Finally, we examined the influence of ocular parameters on the RPE melanin quantification. Multiple parameters, including the diameter and depth of the retinal blood vessel and the RPE layer thickness, may affect PAOM measurement. Based on our simulated results, we ruled out these concerns and validated the feasibility of our calibrated RPE melanin quantification scheme. 2.Methods and Materials2.1.Eye ModelThe posterior eye is often considered to consist of 4 anatomic layers: neural retinal layer, RPE, choroid, and sclera.30–32 In this study, our interest is the RPE, which is around thick. In PAOM, optical absorption and scattering from neighboring layers, especially the highly optically absorbing choroid, will affect the detected PA signals from the RPE. Considering that choroid is not optically uniform, modeling choroid as a homogeneous layer will not provide the most accurate estimation of the RPE melanin in PAOM. We divided the choroid into 3 sublayers: one inner choroidal blood sublayer and 2 outer suprachoroid sublayers. Immediately below the Bruch membrane is the choroidal blood layer, which anatomically consists of choriocapillaris and 2 vascular layers (Haller’s and Sattler’s).33 The inner choroid is thus optically modeled as homogeneous blood.32 Melanin is considered as the major optical absorber in suprachoroid, and the inner suprachoroid has lower melanin concentration than the outer suprachoroid.6 Figure 1 shows the eye model used in our MC simulation. The key parameters are as follows: eyeball diameter: 23.0 mm;34 pupil illumination angle: 2 deg;31 retina thickness: ; RPE thickness: ;30 choroidal blood layer thickness: ; inner suprachoroid layer thickness: ; outer suprachoroid layer thickness: ;33 and sclera layer thickness: .31 We placed a cylindrical blood vessel segment (diameter: ) beneath the retina surface,30 and the vessel wall thickness was set to be 10% of the vessel lumen diameter.35 Except for the finer division of the choroid layer, our eye model is similar to what used in previous studies.30–32,36 The wavelength-dependent optical absorption coefficients, scattering coefficients, and scattering anisotropy are extracted from the literatures6,11,31–33,36–40 and are summarized in Table 1. For the blood vessel, RPE, and the 3 choroid sublayers, we calculated the absorption coefficient based on hemoglobin () and melanin concentration () by and where () is the total hemoglobin concentration; (dimensionless) is the oxygen saturation level; , , and () are the molar extinction coefficients of oxy-hemoglobin, hemoglobin, and melanin, respectively; 64,500 () is the molecular weight of hemoglobin; and () is the melanin concentration. In our simulation, we assumed that ,41 and set the as 90% in blood of retinal vessel segment42 and 95% in choroidal blood.33,36Table 1Optical parameters used in Monte Carlo (MC) simulation.
As a simplified, yet, effective way of modeling, optical absorption within retinal blood and choroid blood layer is contributed only by hemoglobin (); optical absorption within the RPE and the 2 suprachoroidea layers are only from melanin ().36 The wavelength-dependent extinction coefficients are obtained from published data.43,44 We neglected the refractive index difference in all the ocular layers.31,32 We simulated 570 and 584 nm illuminations, where absorption coefficients of the deoxy- and oxy-hemoglobin are the same. Thus, the absorption of the whole blood is independent of , which promises accurate reference signals for calibration in our imaging scheme. 2.2.Monte Carlo SimulationIn the MC simulation of photon transport, multiple photon packets are launched into tissue. Reflection or transmission takes place where there is a difference in the refractive indices. The optical scattering anisotropy and the total interaction coefficient determine the propagation direction and the path length between 2 consecutive photon–tissue interaction events,45 while the ratio of absorption coefficient to the total interaction coefficient (albedo) determines the weight loss of photon packet at the location of interaction. At the location of each interaction event, the new photon packet propagation direction is calculated based on the local scattering anisotropy using the Henyey–Greenstein phase function. A photon packet is terminated when it travels outside the tissue or carries too little weight and does not survive the Russian Roulette rule.45 Distribution of the energy deposition and diffuse reflectance of simulated region converge to the true value with increasing photon packets. The correctness of our homemade MC code was confirmed by comparing with previous published data using the same input.45 In our PAOM simulation, photon packets were launched from the center of the pupil. The initial angle was randomly selected but will statistically follow a uniform distribution within the 2-deg field of view.30,31 Most previous studies considered the posterior eye flat when the illumination angle was sufficiently small. In our study, however, the curvature of the fundus was also considered and a spherical coordinate was used.32 As shown in Fig. 1(a), with the center of the eye ball being defined as the coordinate origin, our recorded region of interest (ROI) angle in the simulation was from 0 deg to 5 deg (2.5 times of the illumination angle); was from 0 deg to 360 deg; and was from retina to sclera. To simulate PAOM, light energy deposition was recorded within the selected ROI. 2.3.Retinal Pigment Epithelium Melanin Concentration MeasurementPA signal amplitude is proportional to the product of Grüneisen coefficient (the variation of Grüneisen coefficient in different retinal tissues is ignored), optical absorption coefficient , and the local optical fluence .25,46 The ratio between PA amplitudes from the RPE () and retinal blood vessel () can be expressed as where can be obtained from PAOM experiments; and are the optical fluences from an RPE region and a nearby vessel, which are similar, though not perfectly identical, given the small NA illumination in PAOM47 and that retina is a thin tissue () with weak optical scattering and absorption. In order to demonstrate the concept, we nevertheless get rid of the fluence terms and simplify Eq. (3) as By combining Eqs. (2) and (4), the RPE melanin concentration can be estimated as where is the oxygen-saturation-dependent and can be calculated by Eq. (1). Both and are constant at a given optical wavelength. Therefore, the measured RPE melanin concentration is proportional to the PA amplitude ratio , if neglecting fluence difference.To verify the feasibility of RPE melanin quantification calibrated by retinal blood measurement in PAOM, we numerically investigated the relationship between and the PA amplitude ratio. We varied the RPE melanin concentration from 20 to , which covers all the reported concentrations.10,11 2.4.Simulation of Energy Deposition and Photoacoustic Signal RatioWe obtained the optical energy deposition distribution in the posterior eye and converted it into PA signal amplitude to simulate PAOM. During simulation, the posterior eye volume was discretized into voxels and optical energy deposition was scored into a 3-dimensional matrix, (where , , denote 3 dimensions in spherical coordinate, respectively). The voxel size was chosen to be , much smaller than the actual system resolution. An arbitrary system axial resolution can be simulated by summing adjacent elements in dimension of . The generated PA signal amplitude is proportional to energy deposition. Therefore, the PA amplitude ratio was obtained by taking the ratio of energy deposition at RPE and retinal blood vessel. Admittedly, factors other than energy deposition also affect PA amplitude. However, by taking the ratio, we can rule out factors that influence PA amplitude at RPE and nearby vessel in a similar way, including, e.g., detection sensitivity and acoustic propagation loss. The effect of ultrasonic detection bandwidth was considered by defining system axial resolution. Though the shape and dimensions of optical absorber affect the generated PA amplitude, they were neglected in this simplified eye model, which contains only multiple homogeneous anatomical layers. 3.Results3.1.Optical Energy Deposition DistributionThe simulated optical energy deposition in each anatomical layer is shown in Fig. 2. Figure 2(a) is a typical cross-sectional image of the distribution with the RPE melanin concentration at . Figure 2(b) shows the one-dimensional, depth-resolved optical absorption profiles extracted from Fig. 2(a) at different lateral positions with respect to the center of the blood vessel. Optical absorption in the retina mainly comes from the blood vessel. In regions outside the retinal blood vessel, the illuminating light does not encounter strong attenuation until reaching the RPE. Despite strong attenuation in the RPE, light can still penetrate into the inner choroid. Figure 2(c) shows a simulated impulse response of a 75-MHz bandwidth ultrasonic detection system. Figure 2(d) is the simulated PA A-lines of the energy deposition profiles in Fig. 2(b) obtained by convolving with impulse response. To calculate the RPE melanin concentration, we divided the simulated field into the vessel region (VR) and the RPE region (RR) [Fig. 3(a)] within the plane. Figure 3(b) shows the depth-resolved profiles of optical energy deposition in VR when we varied the RPE melanin concentration from 20 to . The RPE absorption increases with the increment of RPE melanin concentration, while the choroid absorption decreases. Figure 3(c) is the magnified view of the dashed box. Figure 3(d) shows the depth-resolved profiles of optical energy deposition in RR, where the absorption in retina is negligible. The shapes of the lines are analogous to that in Fig. 3(c). We investigated the influence of the RPE melanin concentration on relative optical energy deposition in different posterior eye layers. Figure 4(a) shows the axial energy deposition distribution in VR, and Fig. 4(b) shows the distribution in RR. In VR, the vessel absorbs around 80% of total incident light energy. In RR, inner choroid absorbs the majority of the light until melanin concentration in RPE increases to around . Light is not able to penetrate through choroidal blood and reach outer choroid even if RPE melanin concentration is really low. 3.2.Influence of Photoacoustic Ophthalmoscopy Axial ResolutionMathematically, an ideal linear dependence of the measured RPE melanin concentration on detected PA signal amplitude ratio (Sec. 2) is valid only when PAOM has infinite axial resolution. In practice, such linear dependence can vary considering the finite size of PAOM axial resolution. To investigate the influence of axial resolution, we integrated simulated energy deposition over a depth range corresponding to a practical axial resolution and tested the relationship between RPE melanin concentration and PA signal amplitude ratio. We varied the axial resolution from to . When PAOM cannot resolve the thick RPE layer, the detected PA signal contains contributions from both the RPE and choroid. Figure 5(a) shows the ratio of RPE-generated PA signal to total signal generated within axial resolution. It indicates that when the RPE melanin concentration is low, the detected signal comes more from the choroid than RPE. We varied the RPE melanin concentrations and investigated its effect on PA signal amplitude ratio, as shown in Fig. 5(b). Note that always involves possible contribution from choroidal blood. The RPE thickness is ; therefore, the PA signal is purely from RPE when the axial resolution is . The ratio increases with RPE melanin concentration. But when the axial resolution is worse than , the ratio becomes less sensitive to the change in RPE melanin. This is because absorption from choroidal blood is involved. 3.3.Influence of Excitation WavelengthIsosbestic wavelengths for oxy-hemoglobin and hemoglobin are better suited for measurement since the blood absorption can be independent of oxygen saturation level. There are multiple wavelengths that satisfy this requirement,43 among them 570 and 584 nm are the best choices because of relatively strong optical absorption. Figure 6 shows the amplitude ratio curves for 570 and 584 nm with the preset axial resolution. There is no significant difference between the 2 wavelengths, especially when the RPE melanin concentration is not extremely high. 3.4.Influence of Anatomical VariationsAnatomical variations in eye are reported among individuals,29,30,34 which may affect measurement. To test the robustness of the RPE melanin quantification by PAOM, we varied the depth and diameter of the retinal blood vessel as well as the RPE thickness in the eye model. The axial resolution was set at . Figure 7(a) shows the relationship between the amplitude ratio curves with varied retinal blood vessel size. Three curves are roughly identical despite variation of vessel diameter. Figure 7(b) shows that the depth of the blood vessel in retina barely affects the ratio curve. This is because of weak absorbing and scattering from retina tissue. Figure 7(c) shows that the RPE thickness would influence the ratio curve. Since the axial resolution of PAOM is not able to resolve RPE layer, the detected PA signal is an integration of energy deposition in RPE and inner choroid. When the RPE melanin concentration is high, the absorption coefficient of RPE is larger than that of inner choroid, a thicker RPE will generate stronger PA signal and lead to a larger amplitude ratio. Otherwise, when RPE melanin concentration is low, the absorption coefficient of choroid is larger; a thicker RPE leads to a weaker PA signal and a smaller amplitude ratio. The 3 curves cross at the same point, where absorption coefficients of 2 layers are the same and the amplitude ratio is almost independent of RPE thickness. 4.Discussion4.1.Retinal Pigment Epithelium Melanin ConcentrationThe simulation provides a moderate reference for possible in vivo RPE melanin concentration. So far, no consistency on the absolute RPE melanin concentration can be found from the literature. In the previous MC ocular simulations, difference in the assumed RPE melanin concentration can be as large as 3 orders of magnitude. Preece and Claridge assumed that the RPE melanin concentration is around ,36 whereas in the work of Guo et al., simulation was conducted with the RPE melanin concentration from 0.8 to .32 Experimental studies by Schmidt and Peisch showed that for normal human, melanin concentration is around in macular region and higher concentration is expected in peripheral regions.11 In a more recent study, Durairaj et al. reported 60 to melanin in human choroid-RPE.10 Both of their measurements were in vitro, yet enucleation of eye and solubilization of melanin were performed before measurements, where the accuracy of results might be affected. In our work, we varied RPE melanin concentration from 20 to , which covered the range of reported data and very likely, physiological condition. The study confirms that the strong optical absorption by the RPE is the foundation of melanin quantification using PAOM. Total light absorption in the RPE is less than choroidal vasculatures unless the RPE melanin concentration reaches around . But the deposited energy density in the RPE is usually higher. This, indeed, allows PAOM to quantify the RPE melanin even though the axial resolution cannot resolve the boundary between the RPE and choroid. 4.2.Retinal Pigment Epithelium Melanin Measurement with Photoacoustic OphthalmoscopyOur proposal is to determine the absolute RPE melanin concentration through the ratio of PA signal amplitudes between the RPE and a nearby blood vessel. As indicated in previous section, this ratio should be linearly related to the RPE melanin concentration, assuming the same optical fluence for retinal blood vessel and RPE, and infinite system resolution. However, in reality, the same fluence assumption only holds true for infinitely thin layers on the top boundary of blood vessels and the uppermost surface of the RPE. For tissue volume deeper than the superficial layers, optical fluences are subjected to a roughly exponential decay governed by Beer’s law. Such decays are not negligible due to strong optical absorption in retinal blood vessel and the RPE. The PA detected signal is a result of integrating energy deposition within a resolution-determined tissue volume. Though the initial optical fluence in blood vessel and the RPE is the same, different decay rates lead to different energy depositions within integration volume.48 Therefore, for any finite system resolution, the linear dependence of amplitudes ratio on RPE melanin concentration can still be affected. In addition, the axial resolution of PAOM is typically ,21 which is not capable of resolving such a thin layer as RPE. This leads to the fact that the detected PA signals from the RPE are a combination of signals from the RPE and the choroid beneath. Both of these effects degrade the linear relationship. Despite influence from uneven optical fluence and finite PAOM axial resolution, the ratio is uniquely determined theoretically and can serve as a good practical measure of the RPE melanin concentration. The range of ratio we got from the simulation agrees with previous published experimental data.27 Though the data are obtained from rats, Durairaj et al.10 show that difference in RPE melanin concentration across different species exists, but generally on the same order of magnitude. Desired wavelengths of PAOM measurement are proposed. Wavelengths of 570 and 584 nm are ideal for PA excitation because the absorption coefficient of blood is independent of . The differences between the amplitude ratio curves for 2 wavelengths are 2.1% when the RPE melanin concentration is below , 5.8% when the RPE melanin concentration is between 200 and , and 9.5% when the RPE melanin concentration is between 400 and . Simulation suggests that the proposed method is robust to variations in retinal blood vessels. 20% change in vessel diameter only results in less than 0.4% difference in curves of amplitude ratio. The variation of RPE thickness will affect the curve significantly, especially when melanin concentration is high. Study shows that RPE thickness is in a narrow range among population, ,29 which is covered by simulation. The RPE thickness can be measured by optical coherence tomography (OCT), which achieves as high depth resolution as .49 Quantification of the RPE melanin concentration by PAOM has several benefits. First, the capability of in vivo measurement makes it suitable for clinical diagnosis. Second, the signal detected in PAOM is directly related to the tissue absorption coefficient, and thus melanin concentration and no complicated modeling are involved. Finally, PAOM, whether integrated with other imaging modalities or not, can provide diagnosis information from multiple aspects. 4.3.Ocular Light Absorption Distribution and Retinal Pigment Epithelium Melanin ConcentrationBesides the quantification method, this study helps to establish a thorough understanding, from light-absorption point of view, of the tissue–photon interaction at posterior segment of human eye, which may provide insight to different roles played by hemoglobin, the RPE, and choroid melanin. The large retinal blood vessels absorb the majority of photons at the surface of retina, whereas choriocapillaris and vascular layers absorb all the light that transmits though the RPE. Compared with the RPE melanin, which is a major photon absorber, the choroidal melanin mostly concentrated in the outer choroid, absorbs barely any light. Lack of interaction with light might explain the constant choroidal melanin content during the life span.6 5.ConclusionWe conducted MC simulation to investigate light propagation and energy deposition in posterior eye, and tested the feasibility of using PAOM to quantify the RPE melanin concentration. The simulation was based on an improved eye model with finer anatomical separation in the choroid. We investigated the change of PA signal amplitude ratio between the RPE and the retinal blood vessel with varied RPE melanin concentrations. The current investigation can provide insights in future experimental research. Though not required, an axial resolution that could resolve the RPE layer is desired for measurement with higher sensitivity. It is better to integrate PAOM with other imaging modality, especially high-resolution OCT to compensate for variation of RPE thickness. AcknowledgmentsThe authors thank Siyu Chen, Dr. Ji Yi, Dr. Biqin Dong, and Christina Chan for insightful comments. The authors would like to acknowledge the generous financial support from NIH under Grant Nos. 1R01EY019951, 1R24EY022883, and 2R01EY019484; and NSF under Grant Nos. CBET-1055379 and DBI-1353952. Wenzhong Liu is supported by the Howard Hughes Medical Institute International Research Fellowship. We also acknowledge the computational resources and staff contributions provided by the Quest High-Performance Computing Facility at Northwestern University. Hao F. Zhang has financial interests in Opticent Health Inc., which, however, did not support this work. ReferencesN. M. Bressler, S. B. Bressler and S. L. Fine,
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