Gaussian beam in two-photon fluorescence imaging of rat brain microvessel

Lingyan Shi; Adrián Rodríguez-Contreras; Robert R. Alfano

doi:10.1117/1.JBO.19.12.126006

9 December 2014 Gaussian beam in two-photon fluorescence imaging of rat brain microvessel

Lingyan Shi, Adrián Rodríguez-Contreras, Robert R. Alfano

Author Affiliations +

Journal of Biomedical Optics, Vol. 19, Issue 12, 126006 (December 2014). https://doi.org/10.1117/1.JBO.19.12.126006

Abstract

The critical optical properties of a Gaussian laser beam in two-photon or multiphoton fluorescence imaging, including the beam spot size, depth of focus, and intensity profile, are investigated for spatially locating nanoscale solutes in and surrounding the microvessels of rat brain.

Citation Download Citation

Lingyan Shi, Adrián Rodríguez-Contreras, and Robert R. Alfano "Gaussian beam in two-photon fluorescence imaging of rat brain microvessel," Journal of Biomedical Optics 19(12), 126006 (9 December 2014). https://doi.org/10.1117/1.JBO.19.12.126006

Published: 9 December 2014

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
5 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY