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13 November 2014 Morphological phenotyping of mouse hearts using optical coherence tomography
Michelle Cua, Eric Lin, Ling Lee, Xiaoye Sheng M.D., Kevin S. K. Wong, Glen F. Tibbits, Mirza Faisal Beg, Marinko V. Sarunic
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Journal of Biomedical Optics, Vol. 19, Issue 11, 116007 (November 2014). https://doi.org/10.1117/1.JBO.19.11.116007
Abstract
Transgenic mouse models have been instrumental in the elucidation of the molecular mechanisms behind many genetically based cardiovascular diseases such as Marfan syndrome (MFS). However, the characterization of their cardiac morphology has been hampered by the small size of the mouse heart. In this report, we adapted optical coherence tomography (OCT) for imaging fixed adult mouse hearts, and applied tools from computational anatomy to perform morphometric analyses. The hearts were first optically cleared and imaged from multiple perspectives. The acquired volumes were then corrected for refractive distortions, and registered and stitched together to form a single, high-resolution OCT volume of the whole heart. From this volume, various structures such as the valves and myofibril bundles were visualized. The volumetric nature of our dataset also allowed parameters such as wall thickness, ventricular wall masses, and luminal volumes to be extracted. Finally, we applied the entire acquisition and processing pipeline in a preliminary study comparing the cardiac morphology of wild-type mice and a transgenic mouse model of MFS.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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Michelle Cua, Eric Lin, Ling Lee, Xiaoye Sheng M.D., Kevin S. K. Wong, Glen F. Tibbits, Mirza Faisal Beg, and Marinko V. Sarunic "Morphological phenotyping of mouse hearts using optical coherence tomography," Journal of Biomedical Optics 19(11), 116007 (13 November 2014). https://doi.org/10.1117/1.JBO.19.11.116007
Published: 13 November 2014
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Cited by 13 scholarly publications.
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KEYWORDS
Heart
Optical coherence tomography
Image segmentation
Tissues
Mouse models
Refraction
Image processing
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