Multispectral imaging in the extended near-infrared window based on endogenous chromophores

Qian Cao; Natalia G. Zhegalova; Steven T. Wang; Walter J. Akers; Mikhail Y. Berezin

doi:10.1117/1.JBO.18.10.101318

12 August 2013 Multispectral imaging in the extended near-infrared window based on endogenous chromophores

Qian Cao, Natalia G. Zhegalova, Steven T. Wang, Walter J. Akers, Mikhail Y. Berezin

Author Affiliations +

Journal of Biomedical Optics, Vol. 18, Issue 10, 101318 (August 2013). https://doi.org/10.1117/1.JBO.18.10.101318

Abstract

To minimize the problem with scattering in deep tissues while increasing the penetration depth, we explored the feasibility of imaging in the relatively unexplored extended near infrared (exNIR) spectral region at 900 to 1400 nm with endogenous chromophores. This region, also known as the second NIR window, is weakly dominated by absorption from water and lipids and is free from other endogenous chromophores with virtually no autofluorescence. To demonstrate the applicability of the exNIR for bioimaging, we analyzed the optical properties of individual components and biological tissues using an InGaAs spectrophotometer and a multispectral InGaAs scanning imager featuring transmission geometry. Based on the differences in spectral properties of tissues, we utilized ratiometric approaches to extract spectral characteristics from the acquired three-dimensional “datacube”. The obtained images of an exNIR transmission through a mouse head revealed sufficient details consistent with anatomical structures.

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Qian Cao, Natalia G. Zhegalova, Steven T. Wang, Walter J. Akers, and Mikhail Y. Berezin "Multispectral imaging in the extended near-infrared window based on endogenous chromophores," Journal of Biomedical Optics 18(10), 101318 (12 August 2013). https://doi.org/10.1117/1.JBO.18.10.101318

Published: 12 August 2013

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KEYWORDS

Tissues

Absorption

Proteins

Near infrared

Chromophores

Multispectral imaging

Indium gallium arsenide

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