High-resolution resonant and nonresonant fiber-scanning confocal microscope

Benno H. W. Hendriks; Walter C. J. Bierhoff; Jeroen J. L. Horikx; Adrien E. Desjardins; Cees A. Hezemans; Gert W. t'Hooft; Gerald W. Lucassen; Nenad Mihajlovic

doi:10.1117/1.3534781

1 February 2011 High-resolution resonant and nonresonant fiber-scanning confocal microscope

Benno H. W. Hendriks, Walter C. J. Bierhoff, Jeroen J. L. Horikx, Adrien E. Desjardins, Cees A. Hezemans, Gert W. t'Hooft, Gerald W. Lucassen, Nenad Mihajlovic

Author Affiliations +

Journal of Biomedical Optics, Vol. 16, Issue 2, 026007 (February 2011). https://doi.org/10.1117/1.3534781

Abstract

We present a novel, hand-held microscope probe for acquiring confocal images of biological tissue. This probe generates images by scanning a fiber-lens combination with a miniature electromagnetic actuator, which allows it to be operated in resonant and nonresonant scanning modes. In the resonant scanning mode, a circular field of view with a diameter of 190 μm and an angular frequency of 127 Hz can be achieved. In the nonresonant scanning mode, a maximum field of view with a width of 69 μm can be achieved. The measured transverse and axial resolutions are 0.60 and 7.4 μm, respectively. Images of biological tissue acquired in the resonant mode are presented, which demonstrate its potential for real-time tissue differentiation. With an outer diameter of 3 mm, the microscope probe could be utilized to visualize cellular microstructures in vivo across a broad range of minimally-invasive procedures.

©(2011) Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation Download Citation

Benno H. W. Hendriks, Walter C. J. Bierhoff, Jeroen J. L. Horikx, Adrien E. Desjardins, Cees A. Hezemans, Gert W. t'Hooft, Gerald W. Lucassen, and Nenad Mihajlovic "High-resolution resonant and nonresonant fiber-scanning confocal microscope," Journal of Biomedical Optics 16(2), 026007 (1 February 2011). https://doi.org/10.1117/1.3534781

Published: 1 February 2011

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
8 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY