Molecular basis for optical clearing of collagenous tissues

Jason M. Hirshburg; Krishnakumar M. Ravikumar; Wonmuk Hwang; Alvin T. Yeh

doi:10.1117/1.3484748

1 September 2010 Molecular basis for optical clearing of collagenous tissues

Jason M. Hirshburg, Krishnakumar M. Ravikumar, Wonmuk Hwang, Alvin T. Yeh

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Journal of Biomedical Optics, Vol. 15, Issue 5, 055002 (September 2010). https://doi.org/10.1117/1.3484748

Abstract

Molecular interactions of optical clearing agents were investigated using a combination of molecular dynamics (MD) simulations and optical spectroscopy. For a series of sugar alcohols with low to high optical clearing potential, Raman spectroscopy and integrating sphere measurements were used to quantitatively characterize tissue water loss and reduction in light scattering following agent exposures. The rate of tissue water loss was found to correlate with agent optical clearing potential, but equivalent tissue optical clearing was measured in native and fixed tissue in vitro, given long-enough exposure times to the polyol series. MD simulations showed that the rate of tissue optical clearing correlated with the preferential formation of hydrogen bond bridges between agent and collagen. Hydrogen bond bridge formation disrupts the collagen hydration layer and facilitates replacement by a chemical agent to homogenize tissue refractive index. However, the reduction in tissue light scattering did not correlate with the agent index of refraction. Our results suggest that a necessary property of optical clearing agents is hyperosmolarity to tissue, but that the most effective agents with the highest rates of optical clearing are a subset with the highest collagen solubilities.

©(2010) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Jason M. Hirshburg, Krishnakumar M. Ravikumar, Wonmuk Hwang, and Alvin T. Yeh "Molecular basis for optical clearing of collagenous tissues," Journal of Biomedical Optics 15(5), 055002 (1 September 2010). https://doi.org/10.1117/1.3484748

Published: 1 September 2010

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