Is there a difference between T- and B-lymphocyte morphology?

Dmitry I. Strokotov; Maxim A. Yurkin; Konstantin V. Gilev; Dirk R. Van Bockstaele; Alfons G. Hoekstra; Nikolay Rubtsov; Valerii Pavlovich Maltsev

doi:10.1117/1.3275471

1 November 2009 Is there a difference between T- and B-lymphocyte morphology?

Dmitry I. Strokotov, Maxim A. Yurkin, Konstantin V. Gilev, Dirk R. Van Bockstaele, Alfons G. Hoekstra, Nikolay Rubtsov, Valerii Pavlovich Maltsev

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Journal of Biomedical Optics, Vol. 14, Issue 6, 064036 (November 2009). https://doi.org/10.1117/1.3275471

Abstract

We characterize T- and B-lymphocytes from several donors, determining cell diameter, ratio of nucleus to cell diameter, and refractive index of the nucleus and cytoplasm for each individual cell. We measure light-scattering profiles with a scanning flow cytometer and invert the signals using a coated sphere as an optical model of the cell and by relying on a global optimization technique. The main difference in morphology of T- and B-lymphocytes is found to be the larger mean diameters of the latter. However, the difference is smaller than the natural biological variability of a single cell. We propose nuclear inhomogeneity as a possible reason for the deviation of measured light-scattering profiles from real lymphocytes from those obtained from the coated sphere model.

©(2009) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Dmitry I. Strokotov, Maxim A. Yurkin, Konstantin V. Gilev, Dirk R. Van Bockstaele, Alfons G. Hoekstra, Nikolay Rubtsov, and Valerii Pavlovich Maltsev "Is there a difference between T- and B-lymphocyte morphology?," Journal of Biomedical Optics 14(6), 064036 (1 November 2009). https://doi.org/10.1117/1.3275471

Published: 1 November 2009

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