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1 May 2009 Morphological studies of living cells using gold nanoparticles and dark-field optical section microscopy
Chia-Wei Lee, Miin-Jang Chen, Ji-Yen Cheng, Pei-Kuen Wei
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Journal of Biomedical Optics, Vol. 14, Issue 3, 034016 (May 2009). https://doi.org/10.1117/1.3147390
Abstract
The morphologic changes of living cells under drug interactions were studied by using 80-nm gold nanoparticles and dark-field optical section microscopy. The gold nanoparticles were coated with poly (L-lysine), which attached to the membranes of various cells by way of electrostatic attractive force. A three-dimensional (3-D) morphological image was obtained by measuring the peak scattering intensities of gold nanoparticles at different focal planes. An algorithm for the reconstruction of 3-D cell morphology was presented. With the measured nanoparticle images and calculations, we show morphologic changes of lung cancer cells under the interaction of cytochalasin D drug at different times.
©(2009) Society of Photo-Optical Instrumentation Engineers (SPIE)
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Chia-Wei Lee, Miin-Jang Chen, Ji-Yen Cheng, and Pei-Kuen Wei "Morphological studies of living cells using gold nanoparticles and dark-field optical section microscopy," Journal of Biomedical Optics 14(3), 034016 (1 May 2009). https://doi.org/10.1117/1.3147390
Published: 1 May 2009
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Cited by 22 scholarly publications.
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KEYWORDS
Nanoparticles
Gold
3D image processing
Scattering
Lung cancer
Microscopes
Glasses
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