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1 January 2009 Multipoint fluorescence correlation spectroscopy with total internal reflection fluorescence microscope
Yu Ohsugi, Masataka Kinjo
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Journal of Biomedical Optics, Vol. 14, Issue 1, 014030 (January 2009). https://doi.org/10.1117/1.3080723
Abstract
We report simultaneous determination of diffusion coefficients at different points of a cell membrane using a multipoint fluorescence correlation spectroscopy (FCS) system. A system carrying seven detection areas in the evanescent field is achieved by using seven optical fibers on the image plane in the detection port of an objective-type total internal reflection FCS (TIR-FCS) system. Fluctuation of fluorescence intensity is monitored and evaluated using seven photomultiplier tubes (PMTs) and a newly constructed multichannel correlator. We demonstrate simultaneous-multipoint FCS, with a 3-μs time resolution, to investigate heterogeneous structures such as cell membranes and membrane-binding molecular dynamics near glass surfaces in live cells.
©(2009) Society of Photo-Optical Instrumentation Engineers (SPIE)
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Yu Ohsugi and Masataka Kinjo "Multipoint fluorescence correlation spectroscopy with total internal reflection fluorescence microscope," Journal of Biomedical Optics 14(1), 014030 (1 January 2009). https://doi.org/10.1117/1.3080723
Published: 1 January 2009
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Cited by 21 scholarly publications.
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KEYWORDS
Fluorescence correlation spectroscopy
Diffusion
Luminescence
Optical fibers
Signal detection
Optical correlators
Proteins
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