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1.IntroductionMeasuring retinal vessel oxygen saturation levels could successfully detect relative hypoxia prior to vision-threatening complications, and therapy could be started early, potentially preventing disease progression. Retinal oximetry measurements may provide additional benefits by allowing the provider to give targeted therapy and to determine the response to treatments using the same technique.1, 2, 3 In an effort to address this need, retinal vessel oximetry was pioneered in 1963 by Hickham prior to the development of the clinical pulse oximeter in the early 1970s.4 Their system used a modified fundus camera that acquired dual quasi-monochromatic fundus photographs. Oxygen saturation was estimated by analyzing the optical density of the vessel images at each wavelength, and using oximetry equations based on the Lambert-Beer Law. This early system was used to demonstrate that the oxyhemoglobin saturations as well as retinal vessel reactivity seen in diabetic patients were abnormal.4, 5 Improvements to this fundus photography technique were made by Cohen and Laing5 and Tiedeman 6 The latter identified the influence of fundus reflectivity on these measurements and attempted to compensate for this and calibrate their measurements.6 In addition to photographic retinal oximeters, systems have been developed that scan monochromatic beams across the retinal vasculature and collect the light that is scattered back out of the eye. The earliest scanning oximeter was developed by Delori,7 and recent advancements have been made by Smith 8 Schweitzer 9 used a diffraction grating that measures the reflected optical spectrum of a retinal blood vessel in increments from . Schweitzer’s group also published a method using three isobestic wavelengths and a measurement wavelength in this region to measure oxygen saturation in reflection.10 Neff applied a multispectral imager to retinal oximetry, illuminating with white light and capturing two or four images simultaneously through bandpass filters.11 A similar two-wavelength retinal oximeter has been evaluated by Stefansson.12 Central vessel glints, variations in fundus reflectivity, oxyhemoglobin intermediates, and red blood cell scattering significantly alter the measurement of optical signals in the eye and subsequent calculation of retinal blood oxygen saturations limiting the clinical use of these methods. 4, 7, 9, 13, 14, 15, 16 We have reported an invasive technique, intravitreal illumination, which allows for rejection of the central vessel glint and improved oximetry by displacing the vessel glint from the trans corneal solid collection angle in the swine eye.17 We recently described the blue-green oximetry (BGO) technique, this approach takes advantage of the shift in the spectral minimum from around as oxyhemoglobin increases from 0 to 100% of the hemoglobin present. BGO does not rely on the simplifications required by the methods used to date in retinal oximetry and is more accurate and more easily calibrated than the Lambert-Beer law–based methods when used in hemoglobin solutions or with intact red blood cells.2, 3 However, until now this new oximetry method has not been tested in vivo. In the following, we use intravitrial illumination of the retina in swine to evaluate BGO in vivo. 2.Surgical Procedure and Animal PreparationIntravitreal surgery was performed on four young (approximately five months old) female American Yorkshire domestic swine, and a series of experiments were performed using intravitreal illumination to measure the retinal blood oxygen saturation. The experimental methods procedure detailed below was performed on each swine. The experimental protocol was approved by the University of Arizona Institutional Animal Care & Use Committee. The swine was administered a general anesthetic mixture consisting of air, nitrogen, oxygen, and isofluorane. Swan-Ganz catheters were placed through the femoral vein and artery to monitor blood pressure and arterial oxygen saturation and mixed venous oxygen saturation . The first catheter tip was placed in the aorta just distal to the heart, and the second catheter tip was placed in the inferior vena cava just proximal to the heart. A peripheral oxygen-saturation monitor was also placed in the rectum of the swine, and a lid speculum was placed in the operative eye. A infusion cannulum was placed through the pars plana and sutured in place. The infusion cannulum was attached to a balanced salt solution gravity feed system to maintain intraocular pressure at of water. A contact vitrectomy lens (Bausch & Lomb, F36202.08, Rochester) was placed onto the cornea using an index-matching (refractive index: ) viscoelastic coupling agent. 3.Data AquisitionFollowing the surgical preparation of the animal, light from a scanning monochromator (Oriel Spectral Luminator, Irvine) was coupled into a fiber optic intravitreal illuminator inserted through the pars plana into the vitreous illuminating the retina. The spectral resolution of the monochromator was . A scientific-grade CCD camera (Hamamatsu Orca-AG) with a 3.3X macrozoom lens was used to image the retina. The peak wavelength of a wavelength band was stepped from , and an image of the target area was acquired at each illumination wavelength. All the retina were interrogated using the wavelengths 420, 430, 440, 460, 480, 490, 500, 510, 521, 532, 540, 545, 548, 552, 555, 558, 561, 565, 570, 575, 580, 590, 600, 615, 630, 645, 660, 680, and . In two animals, additional wavelengths in the blue-green region were 475, 488, 506, and . In order to minimize the 2–17% vessel-diameter change across the cardiac cycle,18, 19, 20 each camera exposure was triggered using a cardiac monitor (Digicare Life Windows, Boynton Beach). By minimizing the effect of vessel pulsation, the associated change in optical path length during the cardiac cycle should also be minimized. Some studies indicate that systemic blood oxygen saturation can vary with cardiac cycle; also this effect is avoided by capturing spectral images at the same point in the cardiac cycle.21 Finally, exposure time was adjusted to <40 ms ( of the cardiac cycle) in an attempt to minimize the blood vessel size variation during exposure. The multispectral image sets were obtained over a region of the illuminated fundus containing a selected vein/artery pair. A dark image (obtained with the shutter closed) was subtracted from each image on acquisition to correct for dark current and external light sources. The measurement series began by setting the inspired respiration mixture to 100% oxygen, 0% medical-grade air. The animal was allowed to stabilize for and a multispectral retinal image set was acquired. The oxyhemoglobin saturation , blood partial pressure of oxygen , blood partial pressure of carbon dioxide , pH, and hematocrit were measured from small blood samples collected from the two Swan-Ganz catheters using an ISTAT Personal Clinical Analyzer (Heska Corporation). The measurement cycle ( stabilization, spectral data acquisition, and blood gas analysis) was repeated at 10% decrements, until a pulse oximeter read . 4.Data Calibration and AnalysisThe data were calibrated for the spectral distribution of our illuminator by measuring the output port of an integrating sphere illuminated with the fiber optic illuminator. Then, the multispectral images were normalized by dividing the image intensities by this calibration data set. The inner wall of the integrating sphere is coated with Spectralon®, a common calibration standard.22 This procedure ensured accurate pig’s eye spectral reflectivity by minimizing any spectral power variations from the illumination system or the detector system. The fundus multispectral image sets were registered to correct for eye motion during data collection, and images were aligned to within a fraction of a pixel using a bicubic spline interpolation in conjunction with a maximum mutual information merit function algorithm.23 5.Oxygen Saturation Calibration StandardAbsolute calibration of the retinal arterial oxyhemoglobin saturation measurement relies on the assumption that large arterial oxygen saturation values are constant throughout the body and that the in the aorta will be the same as the .24, 25 This assumption is supported by the observation that the oxygen uptake from a vessel is based on the surface area-to-volume ratio and the retinal arterial vessels that we analyzed are all much larger than the capillaries where oxygen exchange primarily occurs. 6.Light Path AnalysisThe multispectral retinal images were analyzed to determine the spectral reflectivity of the retina, the spectrum of scattering from blood vessels, the transmission of blood vessels, and the level of integrated scatter within the globe. These light fluxes were then applied to evaluate the oxygen saturation of a large artery and vein using BGO. Our initial motivation was to obtain the single-pass scattered light spectrum through vessels by (i) illuminating behind the vessels to provide a strong single-pass signal and (ii) move the vessel glint to the side of the vessel to minimize its interference with the transmitted signal. In the process of this experiment, we learned the significance of the light scattered from the blood column. Figure 1 shows the set of light paths associated with these intravitreal illumination experiments. The source flux from the light pipe illuminates the fundus from the side, displacing reflected glints from the center of the vessel image well to the side of the vessel. The iris established the solid angle of the flux collected from the fundus. This method simplifies the collected flux from the vessels. Let be the flux incident on a point of interest in the fundus from the light pipe. The globe is also full of singly and multiply scattered light, similar to the inside of an integrating sphere. Let be the scattered flux incident on our point of interest.17 Four sets of ray paths are analyzed in our method. First the flux from the illuminated fundus is given by where is the spectral response of the light reflected from the avascular retina into the collection angle through the vitrious, lens, aqueous humor, cornea, and light collection system. The spectral dependence, such as the wavelength in has been suppressed from all expressions. When the camera collects light from blood vessels, two ray paths are significant. The flux from illumination behind the vessel that transmits through the vessel in single pass isThe illumination behind the vessel comes both from the illumination system, , and from the multiply scattered light within the globe, . is the fraction of the light that is not scattered out of the collection angle by blood in the vessel, the vessel wall, and other structures, and is the fraction of the light that is not absorbed by the blood in the vessel, the vessel wall, and other structures. The light scattered from the vessel is related to the incident fluxby , the portion of the total incident flux that is scattered from the blood in the vessel, the vessel wall, and other structures into the collection angle. Thus, the total flux reflected from or through the center of the vessel into the collection angle, , isthe sum of the transmitted and scattered components.Finally, side illumination produces distinct vessel shadows in the retinal images, clearly displaced from the vessels. The flux collected from the vessel shadows is This shadow is the result of the interaction of the scattered and transmitted light, the retinal reflectivity, and the power terms.These equations, despite being approximations, provide insight into the underlying scattering processes as follows. The ratio of the vessel flux to the adjacent avascular fundus reflectance is Equation 6 is independent of the flux terms, providing a solution expressed in transmission and scatter coefficients.We have approximately determined from another experiment measuring the forward transmission of light through a flow cell in a spectrophotometer.3 The effective path length near the center of cylindrical vein is similar in length to the flow cell. The blood hemoglobin concentration, effective path length, and oxyhemoglobin saturation were set close to the expected values for the swine eye, and the data used to approximate .3 The spectrophotometer values of were compared to the measured value of at each wavelength, recognizing that this approximation neglects scattering and absorption by vessel walls.3 We found the following relation held, The value of was of the total at all wavelengths tested from , demonstrating that, for the wavelengths and the angle of incidence used in these swine, the assumption that is reasonable. Thus, the approximationis valid. Equation 8 is useful because it is a ratio involving transmission and reflectance without any absolute flux terms and is relatively easily determined from the in vivo experimental images. The perivascular reflectance, , was a source of calibration error in previous attempts to measure in vivo retinal oxygen saturation.1, 26The data obtained from this experimental design provide measures of the spectral behavior of the perivascular retinal reflectance . Referring to the light-path analysis from Fig. 1, combining and simplifying Eqs. 1, 4 yields When , as seen when light is highly absorbed by hemoglobin , 3, 27 then Eq. 9 further simplifies,Because the power from the light pipe is normalized at each wavelength, the relative reflectance of the perivascular fundus is given by at wavelengths significantly absorbed by hemoglobin.7.ResultsThe spectra of the measured components, , , and from a retinal vein are shown in Fig. 2 . Each spectrum is a complex combination of the interaction of the source flux and the reflected flux with a specific region of the retinal anatomy. These signals are similar to the retinal reflectance spectra reported by others.14 We used the spectra from the region to calculate the values and . Figure 3 shows the spectra of the perivascular retina , an adjacent retinal artery , and the spectra of . Fluctuations in power in the two empiric signals are removed by dividing by . Because the reflected flux is an integrated signal from the globe, it contains a bulk average of the arterial, capillary, and venous hemoglobin along with pigment signals from the eye. Dividing by significantly reduces signal noise (removing ) while shifting the minimum to the right for arteries and to the left for veins (removing ). For the BGO technique to be useful, the analysis must be consistent with changes in vessel diameter, illumination angle, blood concentration, and oxyhemoglobin saturation. Figure 4 shows the monochromatic image of a region of a swine retina at . One can see the vessel shadows adjacent to and just left of the vessels. The shadow is most apparent where the left-hand bifurcation of the large vein crosses over the artery that is also to the left of the large vein in the image. The minima of the spectra of from the branches of the bifurcated vein and the large confluent vein to the right of the large artery in the image are also shown in Fig. 4. Local differences in retinal metabolism and autoregulation will cause small variations in the oxyhemoglobin saturation found in two veins that feed a larger vein. The confluent vein should have a bulk average of the two feeder vein oxyhemoglobin saturations. The minima shown in Fig. 4 are very similar when scans are taken from markedly different-sized veins with different angles of illumination and the large vein minimum is between the two feeder vein minima as expected. Figure 5 shows how the spectra of for the artery and the vein shown in Fig. 4 have minima that are significantly different from each other and behave as predicted by the BGO technique.2, 3 The minimum of the arterial fit is and the vein is , demonstrating sensitivity to oxygen saturation. In Fig. 6 , we show the relative optical density and retinal reflectivity from a swine retinal artery calculated using Eqs. 8, 10. The BGO minimum shown here is in the region of the spectrum expected for an artery, and the reflectivity of the perivascular fundus is nearly neutral. By selecting the capillary-poor perivascular region and using our light-path analysis to remove the scattered light component , we are able to determine the retinal reflectance . Because light that traverses the retinal pigment epithelium is nearly all absorbed by melanin,14 the return signal , shown in Fig. 6, is nearly all flux that has been scattered back to the detector from the inner retina. This result is consistent with recently published work with a retinal microsurgery isolated in vivo inner retina, showing that the perivascular inner retina has very little absorption and is nearly spectrally neutral.17 On the basis of these observations, we fit a parabola to the plot of for the retinal arteries in four swine and calculated the minima. A calibration plot was generated by comparing these minima to the oxyhemoglobin saturations in the swine arterial circulation. The quality of the calibration plot was evaluated by calculating the standard deviation of the residuals. Figure 7 shows the calibration plot for this in vivo analysis using BGO to analyze 22 measurements in 4 swine with different arterial oxyhemoglobin saturations, vessel diameters and hematocrits (24–45%). The slope of /percent saturation, the intercept of and the standard deviation of the calibration residual error of saturation are similar to BGO in hemoglobin and blood.2, 3 The difference in calibration intercepts from the hemoglobin calibration line to the blood calibration line in transmission is related to the slope of wavelength-dependent scattering and is discussed at length elsewhere.3 The opposite shift in intercepts seen when BGO is used in vivo is likely due to the change in the sign of the slope of wavelength-dependent scattering when a signal is collected in reflection in the retina rather than in transmission with a spectrophotometer.3 Using the calibration line from Fig. 7 to convert minima to saturations, the large vessels shown in Fig. 4 have a retinal venous oxyhemoglobin saturation of 64% and retinal arterial oxyhemoglobin saturation of 98% . 8.Spectral Minimum In Vivo Error SensitivityWe performed multiple measures on vessels in the same region moving up one pixel at a time for five lines. The standard deviation of the minima residuals was or 1% saturation . Though this is not exactly the same as repeated measures at the same position, it is a more accurate test of the sensitivity of the method to error because changes in oxyhemoglobin saturation over time and systematic movements are significant confounders likely more important than the changes that occur over a five-pixel range while one frame of data is being collected. One longstanding problem with retinal oximetry has been the poorly conditioned nature of the equations. Small errors in data lead to far larger errors in the oxygen saturation determinations.1, 28 We evaluated the conditioning of the BGO technique theoretically by assuming a 1% error in each optical density and rereducing the swine reflectance data. A 244-value Gaussian distributed pseudorandom number set with a zero mean and a standard deviation of was generated. Using the spectra from two swine arterioles and a venule with different oxyhemoglobin saturations (96.7, 85, 54%) and three (475, 488, ), four (475, 488, 500, ), five (460, 475, 488, 500, ) and 10 wavelengths (460, 475, 480, 488, 490, 500, 506, 510, 514, ), we measured the standard deviation of the calculated minimum when the 244 errors where added to the spectra. The greatest residual error was seen using the 96.7% oxyhemoglobin saturation data with three wavelengths ( saturation), and the lowest error when 54% oxyhemoglobin saturation data with 10 wavelengths were used ( saturation). 9.Comment on Intravitreal IlluminationIntravitreal illumination is highly invasive, requiring a puncture through the pars plana. It must be performed in a sterile environment by a highly trained practitioner. A vitrectomy is also necessary if one seeks to freely move the light probe through the vitreous chamber. As such, we are not proposing intravitreal illumination as the basis for a widely applicable retinal oximetry test. We do emphasize that the unique light paths provided by intravitreal illumination are particularly useful for developing models of light propagation in the posterior chamber. These model equations take advantage of the reduced specular reflections occurring anterior to the blood column in the vessel and also remove the possibility of light double passing through blood vessels. Using these model equations with information available from other sources allowed us to demonstrate the relative contribution of (return light through the vessel) and (backscattered light from the vessel and its contents) to the return signal from the center of the vessel. The analysis using this unique geometry also allowed us to solve for the spectrum of the perivascular inner retina without using Spectralon, as we had previously done.17 Because the back-reflected light that traversed the vessel and returned to the detector was shown to be negligible when BGO wavelengths were used, removing double-pass light was shown to be unnecessary. Our work here indicates that the exclusion of central glints or specular reflections from anterior to the vessel remains an issue, but an on axis illumination system that allowed for the removal of the glint would lead to a similar set of model equations. 10.Arterial Calibration of Venous MeasurementsThe use of arterial signals to calibrate venous measurements using BGO as done here assumes that the arterial profiles and the vein profiles have the same components. The retinal arteries contain blood, are anatomically in analogous circumstances with the same light paths, have vessel walls that scatter light, are in the same size range, and can have the oxyhemoglobin saturation manipulated so that it reaches the range seen in veins. It will be very difficult to generate a better model for the retinal venous circulation than the retinal arteries. However, we know that, aside from oxyhemoglobin saturation, there is a physiologic anemia on the venous side of the circulation, retinal veins are often larger than retinal arteries and venous vessel walls are thinner than corresponding arterial vessel walls. Our results address some of these concerns directly and others by inference. The sizes and the hematocrits of the blood in the arterial vessels we analyzed varied substantially without causing a significant change in the minima we measured based on these parameters. This makes it unlikely that the differences in size between the arteries and veins or physiologic changes in hematocrit from the arterial to venous circulation will have a significant impact on calibration. We remain uninformed about how different vessel wall thicknesses may influence the backscattered light, but the arterial and venous saturations determined using BGO are reasonable. 11.ConclusionsBGO, described previously in hemoglobin and blood,2, 3 is demonstrated here in vivo in an anesthetized pig using a specialized optical system. BGO is sensitive to oxygen saturation and is robust to errors at the 1% level. The relative contribution of single-pass light, double-pass light by inference, and backscattered light in reflectance retinal spectroscopy was separately measured and reported. 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