Angle-resolved Mueller matrix study of light scattering by B-cells at three wavelengths of 442, 633, and 850  nm

Huafeng Ding; Jun Q. Lu; Robert Scott Brock; Thomas J. McConnelll; Jenifer F. Ojeda; Kenneth Jacobs; Xin-Hua Hu

doi:10.1117/1.2749730

1 May 2007 Angle-resolved Mueller matrix study of light scattering by B-cells at three wavelengths of 442, 633, and 850 nm

Huafeng Ding, Jun Q. Lu, Robert Scott Brock, Thomas J. McConnelll, Jenifer F. Ojeda, Kenneth Jacobs, Xin-Hua Hu

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Journal of Biomedical Optics, Vol. 12, Issue 3, 034032 (May 2007). https://doi.org/10.1117/1.2749730

Abstract

Angle-resolved signals of polarized light scattered by biological cells provide rich information on cell morphology. Quantitative study of these signals can lead to new methods to develop and improve high-throughput instrumentation for cell probing such as scattering-based flow cytometry. We employ a goniometer system with a photoelastic modulation scheme to determine selected Mueller matrix elements of B-cell hydrosol samples. The angular dependence of S₁₁, S₁₂, and S₃₄ is determined from the scattered light signals between 10 and 160 deg at the three wavelengths 442, 633, and 850 nm. A finite-difference, time-domain (FDTD) method and coated-sphere model are used to investigate the effect of nuclear refractive index on the angle-resolved Mueller elements at different wavelengths using the 3-D structures of selected B cells reconstructed from confocal images. With these results, we demonstrate the value of the light-scattering method in obtaining the cell morphology information.

©(2007) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Huafeng Ding, Jun Q. Lu, Robert Scott Brock, Thomas J. McConnelll, Jenifer F. Ojeda, Kenneth Jacobs, and Xin-Hua Hu "Angle-resolved Mueller matrix study of light scattering by B-cells at three wavelengths of 442, 633, and 850 nm," Journal of Biomedical Optics 12(3), 034032 (1 May 2007). https://doi.org/10.1117/1.2749730

Published: 1 May 2007

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