PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
Plant pathogens represent a significant threat to food supplies. Agricultural diagnostics currently function on a paradigm involving either inaccurate visual inspection or burdensome laboratory molecular tests. Several field-ready diagnostic methods have been presented in recent years; however detection of pre-symptomatic or co-occurring infections and in-field sample processing remain challenges. To address these challenges, we developed surface-enhanced Raman scattering (SERS)-sensing hydrogels that uptake pathogenic material (RNA) and produce a measurable response on-site. Our novel reagentless SERS sensor for the detection of tobacco mosaic virus (TMV) was embedded in an environmentally compatible hydrogel material, to produce sensing hydrogels. We demonstrate the diagnostic application of our sensing hydrogels through exposure to TMV infected tobacco plants. This technology offers a field-deployable tool for pre-symptomatic and multiplexed molecular identification of pathogens with the potential to shift the current agricultural diagnostic paradigm.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
The alert did not successfully save. Please try again later.
Lyndsay N. Kissell, Huazhen Liu, Manisha Sheokand, Der Vang, Pradeep Kachroo, Pietro Strobbia, "Sensing hydrogels based on surface-enhanced Raman scattering for the direct detection of plant viruses in infected plants," Proc. SPIE PC13059, Smart Biomedical and Physiological Sensor Technology XXI, PC1305903 (7 June 2024); https://doi.org/10.1117/12.3013882