Microbeads accelerate gellation in 3D collagen hydrogels

Remy Avila; Laura Rodríguez-Mandujano; Reinher Pimentel-Domínguez; Elisa V. Tamariz; Astrid L. Giraldo-Betancur; Edgar Campos-Puente

doi:10.1117/12.3022672

19 June 2024 Microbeads accelerate gellation in 3D collagen hydrogels

Remy Avila, Laura Rodríguez-Mandujano, Reinher Pimentel-Domínguez, Elisa V. Tamariz, Astrid L. Giraldo-Betancur, Edgar Campos-Puente

Author Affiliations +

Proceedings Volume PC13010, Tissue Optics and Photonics III; PC1301007 (2024) https://doi.org/10.1117/12.3022672
Event: SPIE Photonics Europe, 2024, Strasbourg, France

Abstract

Collagen type I is extensively employed in 3D cell culture and tissue engineering.This research investigates the impact of embedded microbeads of different types on the fibrillogenesis process in collagen type I hydrogels. Our findings reveal that the presence of microbeads within the collagen matrix alters the fibrillogenesis dynamics. Specifically, carboxylated fluorescent microbeads accelerate gelation by 3.6 times, while silica microbeads decelerate collagen fibril formation by a factor of 1.9, in comparison to pure collagen hydrogels. These observations suggest that carboxylate microbeads serve as nucleation sites, influencing the binding of early collagen fibrils to the microbeads.

Conference Presentation

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Remy Avila, Laura Rodríguez-Mandujano, Reinher Pimentel-Domínguez, Elisa V. Tamariz, Astrid L. Giraldo-Betancur, and Edgar Campos-Puente "Microbeads accelerate gellation in 3D collagen hydrogels", Proc. SPIE PC13010, Tissue Optics and Photonics III, PC1301007 (19 June 2024); https://doi.org/10.1117/12.3022672

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