Comparative studies on UVA and femtosecond corneal crosslinking characterized using Brillouin microscopy

Christian Alejandro Iriarte Valdez; Johannes Wenzel; Maria Leilani Torres-Mapa; Alexander Heisterkamp; Oliver Stachs; Colette Leyh; Emilie Baron; Alexandra Claus

doi:10.1117/12.3016711

19 June 2024 Comparative studies on UVA and femtosecond corneal crosslinking characterized using Brillouin microscopy

Christian Alejandro Iriarte Valdez, Johannes Wenzel, Maria Leilani Torres-Mapa, Alexander Heisterkamp, Oliver Stachs, Colette Leyh, Emilie Baron, Alexandra Claus

Author Affiliations +

Proceedings Volume PC13010, Tissue Optics and Photonics III; PC1301003 (2024) https://doi.org/10.1117/12.3016711
Event: SPIE Photonics Europe, 2024, Strasbourg, France

Abstract

Corneal crosslinking (CXL) with UVA light is the primary treatment for keratoconus, a disease that affects cornea's stability, transparency and shape. UVA-CXL has limitations in penetration depth and unwanted irradiation on healthy tissue. As an alternative, a near-infrared femtosecond laser was used for targeted corneal crosslinking of fresh pigs’ corneas. Brillouin microscopy was implemented as a non-destructive method to determine the viscoelastic properties, by measuring the Brillouin shift. We compared the Brillouin shifts measured for UVA-CXL and fs-CXL treated corneas. Measurements were also performed on UVA-CXL pure bovine collagen I in order to correlate the changes observed in CXL cornea. An increase in Brillouin shift before and after crosslinking, for both UVA and femtosecond-CXL are measurable. We demonstrate the precision and efficacy of using femtosecond CXL in spatial targeted CXL at depth in corneal tissues.

Conference Presentation

Citation Download Citation

Christian Alejandro Iriarte Valdez, Johannes Wenzel, Maria Leilani Torres-Mapa, Alexander Heisterkamp, Oliver Stachs, Colette Leyh, Emilie Baron, and Alexandra Claus "Comparative studies on UVA and femtosecond corneal crosslinking characterized using Brillouin microscopy", Proc. SPIE PC13010, Tissue Optics and Photonics III, PC1301003 (19 June 2024); https://doi.org/10.1117/12.3016711

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available