Phase shifting common path polarization grating based diffraction tomography

Piotr Zdankowski; Julianna Winnik; Mikolaj Rogalski; Marcin Marzejon; Emilia Wdowiak; Maciej Trusiak

doi:10.1117/12.3022319

18 June 2024 Phase shifting common path polarization grating based diffraction tomography

Piotr Zdankowski, Julianna Winnik, Mikolaj Rogalski, Marcin Marzejon, Emilia Wdowiak, Maciej Trusiak

Proceedings Volume PC12996, Unconventional Optical Imaging IV; PC129960N (2024) https://doi.org/10.1117/12.3022319
Event: SPIE Photonics Europe, 2024, Strasbourg, France

Abstract

This research presents the WUTScope, a novel interferometric microscope developed by the Quantitative Computational Imaging group at Warsaw University of Technology. This system, leveraging Quantitative Phase Microscopy and Optical Diffraction Tomography, provides insightful three-dimensional reconstructions of the refractive index distribution in semi-transparent objects. The WUTScope is distinguished by its compact design and capability to operate under partially coherent illumination, using polarization diffraction gratings for beam splitting and recombination. This approach allows for efficient phase shifting and reduces speckle noise, enhancing image signal-to-noise ratio. The system's achromatic nature, due to the identical optical paths of the diffraction orders, facilitates the use of less coherent light sources, a distinct advantage over traditional holographic methods. Its effectiveness is demonstrated through tomographic reconstruction of a 3D-printed brain sample and analysis of refractive index changes in HeLa cells' lipid droplets, revealing the impact of cholesterol accumulation.

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Citation Download Citation

Piotr Zdankowski, Julianna Winnik, Mikolaj Rogalski, Marcin Marzejon, Emilia Wdowiak, and Maciej Trusiak "Phase shifting common path polarization grating based diffraction tomography", Proc. SPIE PC12996, Unconventional Optical Imaging IV, PC129960N (18 June 2024); https://doi.org/10.1117/12.3022319

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KEYWORDS

Diffraction

Tomography

Phase shifting

Polarization gratings

Light sources and illumination

Cameras

Phase distribution

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