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13 March 2024 Genetically encoded sensors to probe environmental hydrophobicity based on ultrafast fluorescence anisotropy changes in green fluorescent proteins
Alejandro Sanchez-Pedreno Jimenez, Youngchan Kim, Henry L. Puhl III, Steven S. Vogel
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Proceedings Volume PC12863, Quantum Effects and Measurement Techniques in Biology and Biophotonics; PC128630W (2024) https://doi.org/10.1117/12.3001754
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
Quantum mechanics has significantly advanced our understanding of fundamental properties. While biological studies have traditionally been assumed to be governed by classical physics, recent studies have shown that coherent excitonic coupling between two chromophores in a homodimer of the yellow fluorescent protein, Venus, is possible at room temperature [Y. Kim et al., Biophys. J., 2019, 116, 1918-1930]. In this study, we present a genetically encoded sensor, inspired by recent finding of the ultrafast photoinduced energy transfer between dimeric enhanced green fluorescent proteins (dEGFPs), to monitor hydrophobic environmental changes [A. Sanchez-Pedreno Jimenez, et al., Phys. Chem. Chem. Phys., in press]. This sensor can be used to investigate the spatial and temporal dynamics biocondensates in cells. This biosensor offers an innovative approach to unravel the dynamics of biocondensates, to elucidate their biological functions and potential implications in health and disease. Experiments are being conducted to test this sensor in living cells.
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Alejandro Sanchez-Pedreno Jimenez, Youngchan Kim, Henry L. Puhl III, and Steven S. Vogel "Genetically encoded sensors to probe environmental hydrophobicity based on ultrafast fluorescence anisotropy changes in green fluorescent proteins", Proc. SPIE PC12863, Quantum Effects and Measurement Techniques in Biology and Biophotonics, PC128630W (13 March 2024); https://doi.org/10.1117/12.3001754
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KEYWORDS
Quantum sensors
Green fluorescent proteins
Ultrafast phenomena
Environmental sensing
Fluorescence anisotropy
Quantum encoding
Biosensors
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