CONFERENCE PROCEEDINGS
Advanced Search
Home > Proceedings > Volume PC12855 > Article
Presentation
13 March 2024 Nanoscale structural mapping of protein aggregates in live cells modeling Huntington's disease by mid-infrared photothermal microscopy
Zhongyue Guo, Giulio Chiesa, Jiaze Yin, Adam Sanford, Stefan Meier, Ahmad S. Khalil, Ji-Xin Cheng
Author Affiliations +
Proceedings Volume PC12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024; PC128550Z (2024) https://doi.org/10.1117/12.3000552
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
Protein aggregation is associated with many neurodegenerative diseases. We applied mid-infrared photothermal (MIP) to dissect the secondary structure of protein aggregates in living yeast cells modeling Huntington’s disease. We validated that MIP spectroscopy could reveal β-sheet enrichment by significant spectral changes and confirmed the β-sheet enrichment of aggregates in high throughput by wide-field fluorescence-detected MIP. By label-free identification of protein aggregates, we observed a further red shift in spectra for aggregates without GFP tag. Finally, by performing MIP spectroscopy with fine spatial steps, we discovered a partition of secondary structures between β-rich core and ɑ-rich shell of the aggregates.
Conference Presentation
© (2024) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation Download Citation
Zhongyue Guo, Giulio Chiesa, Jiaze Yin, Adam Sanford, Stefan Meier, Ahmad S. Khalil, and Ji-Xin Cheng "Nanoscale structural mapping of protein aggregates in live cells modeling Huntington's disease by mid-infrared photothermal microscopy", Proc. SPIE PC12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024, PC128550Z (13 March 2024); https://doi.org/10.1117/12.3000552
ACCESS THE FULL ARTICLE
ORGANIZATIONAL
Sign in with credentials provided by your organization.
INSTITUTIONAL
Select your institution to access the SPIE Digital Library.
SELECT YOUR INSTITUTION
PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
PERSONAL SIGN IN
No SPIE Account? Create one
PURCHASE THIS CONTENT
SUBSCRIBE TO DIGITAL LIBRARY
50 downloads per 1-year subscription
Members: $195
Non-members: $335 ADD TO CART
25 downloads per 1 - year subscription
Members: $145
Non-members: $250 ADD TO CART
PURCHASE SINGLE ARTICLE
Includes PDF, HTML & Video, when available
Members: $17.00
Non-members: $21.00 ADD TO CART
PROCEEDINGS
 PRESENTATION
WATCH
PRESENTATION SAVE TO MY LIBRARY
GET CITATION
Advertisement
Advertisement
KEYWORDS
Proteins
Mid-IR
Neurological disorders
Associative arrays
Diseases and disorders
Optical microscopy
Spatial resolution
RELATED CONTENT
Diagnosis and treatment for Huntington’s disease
Proceedings of SPIE (September 07 2023)
Bond-selective phase contrast imaging (Conference Presentation)
Proceedings of SPIE (January 01 1900)
Nonlinear optics improves mid-infrared vibrational imaging
Proceedings of SPIE (January 01 1900)
A new large well 1024x1024 Si As detector for the...
Proceedings of SPIE (September 13 2005)
Noninvasive measurement of moisture distribution in individual wheat kernels by...
Proceedings of SPIE (January 06 1995)
Study of melanoma invasion by FTIR spectroscopy
Proceedings of SPIE (February 29 2008)
Near-Infrared Magnetic Circular Dichroism Of Heme Proteins
Proceedings of SPIE (May 08 1989)
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top