Time-resolved polychromatic digital holographic microscopy: a quasi-coherent noise free technique for live-cell imaging

Céline Larivière-Loiselle; Mohamed Haouat; Erik Bélanger; Pierre Marquet

doi:10.1117/12.3000627

13 March 2024 Time-resolved polychromatic digital holographic microscopy: a quasi-coherent noise free technique for live-cell imaging

Céline Larivière-Loiselle, Mohamed Haouat, Erik Bélanger, Pierre Marquet

Author Affiliations +

Proceedings Volume PC12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024; PC128540S (2024) https://doi.org/10.1117/12.3000627
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

We have developed Polychromatic Digital Holographic Microscopy (P-DHM), providing quasi coherent noise-free OPD images, but with some limitations regarding the acquisition and reconstruction time of the multi-wavelength holograms. Our recent work, enabling a fully automated multi-wavelength hologram reconstruction associated with an increased acquisition speed, achieving a frame rate between 6 and 15 fps, while preserving OPD high sensitivity, have led to a P-DHM efficient for live-cell imaging. In addition to these technical developments, appealing applications such as the exploration of intracellular trafficking, cell migration, and neurite evolution achieved with P-DHM will be presented.

Conference Presentation

Citation Download Citation

Céline Larivière-Loiselle, Mohamed Haouat, Erik Bélanger, and Pierre Marquet "Time-resolved polychromatic digital holographic microscopy: a quasi-coherent noise free technique for live-cell imaging", Proc. SPIE PC12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024, PC128540S (13 March 2024); https://doi.org/10.1117/12.3000627

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