Dual excitation autofluorescence lifetime and reflectance imaging for fast multidimensional characterization of tissues

Alberto I. Herrando; Vladislav Shcheslavskiy; Riccardo Cicchi; Pedro G. Silva; Joao Lagarto

doi:10.1117/12.3002497

13 March 2024 Dual excitation autofluorescence lifetime and reflectance imaging for fast multidimensional characterization of tissues

Alberto I. Herrando, Vladislav Shcheslavskiy, Riccardo Cicchi, Pedro G. Silva, Joao Lagarto

Author Affiliations +

Proceedings Volume PC12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024; PC1285404 (2024) https://doi.org/10.1117/12.3002497
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

We report a fiber-based imaging system that combines autofluorescence and reflectance spectroscopy for rapid and multidimensional characterization of tissues. The system utilizes dual wavelength excitation and time-resolved fluorescence data to assess tissue properties in real-time. Initial experiments using human cancer specimens demonstrated the system's effectiveness in providing multiparametric tissue characterization, which could improve diagnostic and therapeutic approaches. The integration of the system with a commercial endoscope further emphasizes its clinical applicability and viability. This work showcases a potential pathway for translating this technology into clinical settings, enabling objective and quantitative evaluation of patients in real-time for diagnostic and follow-up.

Conference Presentation

Citation Download Citation

Alberto I. Herrando, Vladislav Shcheslavskiy, Riccardo Cicchi, Pedro G. Silva, and Joao Lagarto "Dual excitation autofluorescence lifetime and reflectance imaging for fast multidimensional characterization of tissues", Proc. SPIE PC12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024, PC1285404 (13 March 2024); https://doi.org/10.1117/12.3002497

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Tissues

Autofluorescence

Reflectivity

Biological imaging

Diagnostics

Reflectance spectroscopy

Signal processing

Show All Keywords

Keywords/Phrases

Search In:

Publication Years