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13 March 2024 A versatile Fourier light field microscopy platform for multi-purpose bio-imaging
Yangyang Bai, Matthew Jones, Sebastian Ojeda, Janielle Cuala, Lynne Cherchia, Scott E. Fraser, Thai V. Truong
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Proceedings Volume PC12848, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI; PC1284806 (2024) https://doi.org/10.1117/12.3002009
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
Fourier light field microscopy (FLFM) captures a sample 3D volume in a single snapshot, providing game-changing imaging speed for various bio-imaging applications. Existing FLFM platforms have often been designed as single-purpose implementations, with a fixed performance in resolution and field of view, restricting widespread applications. Here, to democratize FLFM toward broader adoption for biomedical research, we describe a multi-purpose implementation of FLFM that enables synchronous volumetric imaging across a wide range of resolution and field of view. With our single instrument, we demonstrate a variety of bio-imaging applications across scales, including sub-cellular dynamics, tissue cellular dynamics, and whole-brain neural activity.
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(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.
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Yangyang Bai, Matthew Jones, Sebastian Ojeda, Janielle Cuala, Lynne Cherchia, Scott E. Fraser, and Thai V. Truong "A versatile Fourier light field microscopy platform for multi-purpose bio-imaging", Proc. SPIE PC12848, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI, PC1284806 (13 March 2024); https://doi.org/10.1117/12.3002009
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KEYWORDS
Microscopy
Biological samples
Biological imaging
Biomedical applications
Biomedical optics
Cameras
Image resolution
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