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13 March 2024 Wide-field real-time broadband CARS microscopy
Chiara Ceconello, Andrea Rabolini, Federico Vernuccio, Arianna Bresci, Francesco Manetti, Salvatore Sorrentino, Renzo Vanna, Giulio Cerullo, Dario Polli
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Proceedings Volume PC12847, Multiphoton Microscopy in the Biomedical Sciences XXIV; PC128470J (2024) https://doi.org/10.1117/12.3004526
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
Non-linear optical (NLO) microscopy techniques like coherent anti-Stokes Raman scattering (CARS) are highly effective tools for label-free vibrational imaging, allowing for chemical analysis of biological samples in their native state. We introduce video-rate wide-field CARS microscopy over a vast field of view (tens of micrometers) to enable real-time analysis of fast biological dynamics with down to few-millisecond time resolution. We generate stable broadband Stokes pulses in a YAG crystal using an amplified ytterbium laser source delivering 260-fs high-energy (μJ-level) pulses in the near-infrared. Our system combines fast and tunable single-wavelength real-time imaging with the acquisition of hypercubes, chemically more information-rich.
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Chiara Ceconello, Andrea Rabolini, Federico Vernuccio, Arianna Bresci, Francesco Manetti, Salvatore Sorrentino, Renzo Vanna, Giulio Cerullo, and Dario Polli "Wide-field real-time broadband CARS microscopy", Proc. SPIE PC12847, Multiphoton Microscopy in the Biomedical Sciences XXIV, PC128470J (13 March 2024); https://doi.org/10.1117/12.3004526
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KEYWORDS
Microscopy
Vibration
Nonlinear optics
Pulse signals
Raman spectroscopy
Signal generators
Supercontinuum generation
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