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13 March 2024 PLμTO: a polarized light-sheet microscopy and tomography system for versatile multi-scale and high-throughput cellular resolution imaging
Oscar R. Benavides, Alex J. Walsh
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Proceedings Volume PC12827, Multiscale Imaging and Spectroscopy V; PC128270Q (2024) https://doi.org/10.1117/12.3001188
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
We present a polarized light-sheet microscopy and tomography system, PLμTo, for versatile multi-scale and multi-modal volumetric optical imaging. The system can utilize fluorescence and elastic scattering contrast for visualization of biochemical and structural information. PLμTo is compatible with traditional slide and well-plate sample mounting, as well as microfluidics for high-throughput volumetric imaging flow cytometry of mesenchymal stem cells cultured on spherical microcarriers in a bioreactor culture. Additionally, the system is capable of rapidly screening millimeter-sized cleared tissues with cellular level resolution in a few minutes. This system can be utilized to study biological processes at varying spatial and temporal scales.
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Oscar R. Benavides and Alex J. Walsh "PLμTO: a polarized light-sheet microscopy and tomography system for versatile multi-scale and high-throughput cellular resolution imaging", Proc. SPIE PC12827, Multiscale Imaging and Spectroscopy V, PC128270Q (13 March 2024); https://doi.org/10.1117/12.3001188
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KEYWORDS
Imaging systems
Biological imaging
Image resolution
Pluto
Polarized microscopy
Tomography
Biological samples
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