Variable metalens-based optical sectioning fluorescence microscopy with HiLo algorithm

Cheng Hung Chu; Yu-Hsin Chia; Mu Ku Chen; Sunil Vyas; Din Ping Tsai; Yuan Luo

doi:10.1117/12.2676659

5 October 2023 Variable metalens-based optical sectioning fluorescence microscopy with HiLo algorithm

Cheng Hung Chu, Yu-Hsin Chia, Mu Ku Chen, Sunil Vyas, Din Ping Tsai, Yuan Luo

Proceedings Volume PC12648, Plasmonics: Design, Materials, Fabrication, Characterization, and Applications XXI; PC126480L (2023) https://doi.org/10.1117/12.2676659
Event: SPIE Nanoscience + Engineering, 2023, San Diego, California, United States

Abstract

We present the use of Moiré metalens in fluorescence microscopy system combining with telecentric system and HiLo algorithm for bio-imaging. The Moiré metalens is composed of paired phase metasurfaces. The system is capable of tuning the focal length from 10 mm to 125 mm by tuning the relative angle between the paired metasurfaces without axial displacement. In addition, the speckle illumination HiLo microscopy is used to mitigate the out-of-focus light scattering, the background noise. The labeled beads and ex vivo mouse intestinal tissue samples are imaged to show the optical sectioning capacity of our system. It is anticipated that the proposed design of varifocal metalens would have significant applications in fluorescence microscopy and endoscopy.

Conference Presentation

Citation Download Citation

Cheng Hung Chu, Yu-Hsin Chia, Mu Ku Chen, Sunil Vyas, Din Ping Tsai, and Yuan Luo "Variable metalens-based optical sectioning fluorescence microscopy with HiLo algorithm", Proc. SPIE PC12648, Plasmonics: Design, Materials, Fabrication, Characterization, and Applications XXI, PC126480L (5 October 2023); https://doi.org/10.1117/12.2676659

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Fluorescence microscopy

Lenses

Microscopy

Endoscopy

Light scattering

Light sources and illumination

Monochromatic aberrations

Show All Keywords

Keywords/Phrases

Search In:

Publication Years