In situ lipid metabolism characterization in cells and brain organoids by optical photothermal infrared microscopy (Conference Presentation)

Yeran Bai; Stella Glasauer; Carolina M. Camargo; Xinran Tian; Andrew Longhini; Kenneth Kosik; Craig Prater

doi:10.1117/12.2651007

15 March 2023 In situ lipid metabolism characterization in cells and brain organoids by optical photothermal infrared microscopy (Conference Presentation)

Yeran Bai, Stella Glasauer, Carolina M. Camargo, Xinran Tian, Andrew Longhini, Kenneth Kosik, Craig Prater

Author Affiliations +

Proceedings Volume PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023; PC123920P (2023) https://doi.org/10.1117/12.2651007
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

We developed an optical infrared photothermal (OPTIR) metabolic imaging platform that enables in situ lipid metabolism characterization at sub-cellular resolution. The cell-type specific lipid metabolic imaging was achieved through the fluorescence module integrated into the OPTIR system. We successfully imaged newly-synthesized lipids after azide-palmitic acid treatment in cells across different model systems including neuroglioma, human-induced pluripotent stem cells (iPSCs), iPSC-derived microglia cells, and iPSC-derived brain organoids. The metabolic heterogeneity in different cell types in brain organoids was observed. Collectivity, the proposed metabolic imaging platform opens a vast potential for in situ metabolism characterization at the single-cell level.

Conference Presentation

Citation Download Citation

Yeran Bai, Stella Glasauer, Carolina M. Camargo, Xinran Tian, Andrew Longhini, Kenneth Kosik, and Craig Prater "In situ lipid metabolism characterization in cells and brain organoids by optical photothermal infrared microscopy (Conference Presentation)", Proc. SPIE PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023, PC123920P (15 March 2023); https://doi.org/10.1117/12.2651007

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